Characterisation of a bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) from Drosophila melanogaster.

  title={Characterisation of a bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) from Drosophila melanogaster.},
  author={Lucinda Winward and William G. F. Whitfield and Timothy J. Woodman and Alexander G McLennan and Stephen T. Safrany},
  journal={The international journal of biochemistry & cell biology},
  volume={39 5},
The intracellular functions of diadenosine polyphosphates are still poorly defined. To understand these better, we have expressed and characterized a heat stable, 16.6kDa Nudix hydrolase (Apf) that specifically metabolizes these nucleotides from a Drosophila melanogaster cDNA. Apf always produces an NTP product, with substrate preference depending on pH and… CONTINUE READING