Characterisation of Shiga toxin-producing Escherichia coli (STEC) isolated from seafood and beef.

@article{Kumar2004CharacterisationOS,
  title={Characterisation of Shiga toxin-producing Escherichia coli (STEC) isolated from seafood and beef.},
  author={Hareesh R S Kumar and I Karunasagar and Tsukamoto Teizou and Kensuke Shima and Shinji Yamasaki},
  journal={FEMS microbiology letters},
  year={2004},
  volume={233 1},
  pages={173-8}
}
Shiga toxin-producing Escherichia coli (STEC) strains isolated in Mangalore, India, were characterised by bead-enzyme-linked immunosorbent assay (bead-ELISA), Vero cell cytotoxicity assay, PCR and colony hybridisation for the detection of stx1 and stx2 genes. Four strains from seafood, six from beef and one from a clinical case of bloody diarrhoea were positive for Shiga toxins Stx1 and Stx2 and also for stx1and stx2 genes. The seafood isolates produced either Stx2 alone or both Stx1 and Stx2… CONTINUE READING