Characterisation of 5-HT3 recognition sites in membranes of NG 108-15 neuroblastoma-glioma cells with [3H]ICS 205-930

@article{Neijt2004CharacterisationO5,
  title={Characterisation of 5-HT3 recognition sites in membranes of NG 108-15 neuroblastoma-glioma cells with [3H]ICS 205-930},
  author={Hans Ch Neijt and Angela Karpf and Philippe Schoeffter and G{\"u}nter Engel and Daniel Hoyer},
  journal={Naunyn-Schmiedeberg's Archives of Pharmacology},
  year={2004},
  volume={337},
  pages={493-499}
}
  • H. Neijt, A. Karpf, D. Hoyer
  • Published 1 May 1988
  • Biology, Chemistry
  • Naunyn-Schmiedeberg's Archives of Pharmacology
Summary1.The binding characteristics of [3H]ICS 205-930, a potent and selective 5-hydroxytryptamine 5-HT3 receptor antagonist, were investigated in membranes prepared from murine neuroblastoma-glioma NG 108-15 cells.2.[3H]ICS 205-930 bound rapidly, reversibly and stereoselectively to a homogeneous population of high affinity recognition sites: Bmax = 58 ± 3 fmol/mg protein, pKD = 9.01 ± 0.08 (n = 11). Non linear regression and Scatchard analysis of saturation data suggested the existence of a… 

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