Characterisation and Expression Analysis of MrLip1, a Class 3 Family Lipase of Malassezia restricta

  title={Characterisation and Expression Analysis of MrLip1, a Class 3 Family Lipase of Malassezia restricta},
  author={Minji Park and Won Hee Jung and Song Hee Han and Young Hoon Lee and Yang Won Lee},
The genus Malassezia is associated with a wide range of skin diseases and is the predominant fungal genus isolated from human skin. Of the 14 Malassezia species identified, M. restricta is the most abundant fungal species found from both healthy and diseased skin. Emerging evidences have suggested that extracellular lipases of Malassezia play a critical role in its survival on the host skin surface. This study aimed to characterise the lipase 1 homologue (MrLip1) in M. restricta and to analyse… 

pH-Dependent Expression, Stability, and Activity of Malassezia restricta MrLip5 Lipase

The results suggest that the activity and expression of MrLip5 are pH-dependent, and that this lipase may play an essential role at the M. restricta-host interface during disease progression.

Skin Commensal Fungus Malassezia and Its Lipases

The existing knowledge on Malassezia, diseases associated with the fungus, and the role of the reported lipases in its physiology and pathogenesis are summarized and an emerging interest for lipases is summarized.

A Novel Virus Alters Gene Expression and Vacuolar Morphology in Malassezia Cells and Induces a TLR3-Mediated Inflammatory Immune Response

This is the first study to identify and characterize a novel mycovirus in Malassezia and show that viral nucleic acid from MrV40 induces a TLR3-mediated inflammatory immune response in bone marrow-derived dendritic cells, indicating that a viral element likely contributes to the pathogenicity of MalasseZia.

Whole genome sequencing analysis of the cutaneous pathogenic yeast Malassezia restricta and identification of the major lipase expressed on the scalp of patients with dandruff

The genome of a clinical isolate of M. restricta was sequence and analysed to search for lipase orthologues and identify the lipase that is the most frequently expressed on the scalp of patients with dandruff, and it was found that the gene encoding lipase homolog MRES_03670, named LIP5 in this study, was the mostrequently expressed lipase in the swab samples.

Ambient pH regulates secretion of lipases in Malassezia furfur.

The qPCR results revealed that a set of functional lipase genes, LIP3-6, were constitutively expressed regardless of pH conditions or exposure time, concluding that the external pH plays a promotional role in the secretion of lipases but exerts less effect on transcription of the genes and morphology in M. furfur.

Fungal Screening on Olive Oil for Extracellular Triacylglycerol Lipases: Selection of a Trichoderma harzianum Strain and Genome Wide Search for the Genes

The success in identifying a lipase gene inducible with olive oil and the suitability of the functional screening and bioinformatic survey carried out herein, support the premise that the strategy can be used in other microorganisms with sequenced genomes to search for true lipases, or other enzymes belonging to large protein families.

Identification and Evaluation of Inhibitors of Lipase from Malassezia restricta using Virtual High-Throughput Screening and Molecular Dynamics Studies

A structure-based virtual screening in Zinc database to find the natural bioactive inhibitors of Mrlip1 and identify three compounds bearing superior affinity and specificity from the Traditional Chinese Medicine database that might act as promising lead drugs againstMrlip1.

Antifungal Mechanism of Action of Lauryl Betaine Against Skin-Associated Fungus Malassezia restricta

Lauryl betaine showed the most potent antifungal activity against Malassezia restricta and the mechanism of action was studied mainly using Cryptococcus neoformans, where the results indicated that the compound mainly affected cell membrane synthesis, particularly ergosterol synthesis.

A Novel Virus Alters Gene Expression and Vacuolar Morphology in <italic toggle='yes'>Malassezia</italic> Cells and Induces a TLR3-Mediated Inflammatory Immune Response

A novel virus alters gene expression and vacuolar morphology in Malassezia cells and induces a TLR3-mediated inflammatory immune response in bone marrow-derived dendritic cells, suggesting that a viral element contributes to the pathogenicity of MalasseZia.



MfLIP1, a gene encoding an extracellular lipase of the lipid-dependent fungus Malassezia furfur.

This is the first molecular description of a secreted lipase from M. furfur, and shows high sequence similarity to other known extracellular lipases, but is not a member of a lipase gene family in M.furfur.

The lipolytic enzymes activities of Malassezia species.

The data suggest that lipase may be a pathogenic factor in the skin disease associated with Malassezia and provide an explanation as to why M. globosa is an important pathogenic species in several human skin diseases despite its slow rate of growth.

Isolation and expression of a Malassezia globosa lipase gene, LIP1.

It is reported that lipase activity was detected in four species of Malassezia, including M. globosa, the first step in a molecular description of lipid metabolism on the scalp, ultimately leading toward a test of its role in D/SD etiology.

Evaluation of Expression of Lipases and Phospholipases of Malassezia restricta in Patients with Seborrheic Dermatitis

Background Malassezia species (spp.) are cutaneous opportunistic pathogens and associated with various dermatological diseases including seborrheic dermatitis, dandruff and atopic dermatitis. Almost

Expression and Characterization of a Novel Lipase from Aspergillus fumigatus with High Specific Activity

A novel lipase gene from Aspergillus fumigatus, afl1-1, was cloned and expressed with a molecular mass of 38 kDa in Escherichia coli for the first time and exhibited potent hydrolysis activity that had not been observed.

Isolation, characterization and molecular cloning of a lipolytic enzyme secreted from Malassezia pachydermatis.

Homology investigations showed that the enzyme showed high esterase activity toward p-nitrophenyl octanoate has similarities principally with 11 lipases produced by Candida albicans and some other yeast lipases.

Secreted lipases of Candida albicans: cloning, characterisation and expression analysis of a new gene family with at least ten members

Data indicate lipid-independent, highly flexible in vitro and in vivo expression of a large number of LIP genes, possibly reflecting broad lipolytic activity, which may contribute to the persistence and virulence of C. albicans in human tissue.

Cloning and expression analysis of NhL1, a gene encoding an extracellular lipase from the fungal pea pathogen Nectria haematococca MP VI (Fusarium solani f. sp. pisi) that is expressed in planta

In vitro, the lipase gene shows a low basal expression, but is highly inducible by lipase substrates, and repressed by glucose, while in vitro growth of N. haematococca shows maximal activity at 37°C and pH 8.0.

A secreted lipase of Fusarium graminearum is a virulence factor required for infection of cereals.

Transformation-mediated disruption of FGL1 led to reduced extracellular lipolytic activity in culture and to reduced virulence to both wheat and maize, which allowed us to isolate, clone, and characterize a gene (FGL1) encoding a secreted lipase.