Characterisation and Expression Analysis of MrLip1, a Class 3 Family Lipase of Malassezia restricta

@article{Park2015CharacterisationAE,
  title={Characterisation and Expression Analysis of MrLip1, a Class 3 Family Lipase of Malassezia restricta},
  author={Minji Park and Won Hee Jung and Song Hee Han and Young Hoon Lee and Yang Won Lee},
  journal={Mycoses},
  year={2015},
  volume={58}
}
The genus Malassezia is associated with a wide range of skin diseases and is the predominant fungal genus isolated from human skin. Of the 14 Malassezia species identified, M. restricta is the most abundant fungal species found from both healthy and diseased skin. Emerging evidences have suggested that extracellular lipases of Malassezia play a critical role in its survival on the host skin surface. This study aimed to characterise the lipase 1 homologue (MrLip1) in M. restricta and to analyse… 
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Background Citations
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Methods Citations
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Results Citations
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11 Citations

pH-Dependent Expression, Stability, and Activity of Malassezia restricta MrLip5 Lipase

The results suggest that the activity and expression of MrLip5 are pH-dependent, and that this lipase may play an essential role at the M. restricta-host interface during disease progression.

Skin Commensal Fungus Malassezia and Its Lipases

The existing knowledge on Malassezia, diseases associated with the fungus, and the role of the reported lipases in its physiology and pathogenesis are summarized and an emerging interest for lipases is summarized.

A Novel Virus Alters Gene Expression and Vacuolar Morphology in Malassezia Cells and Induces a TLR3-Mediated Inflammatory Immune Response

This is the first study to identify and characterize a novel mycovirus in Malassezia and show that viral nucleic acid from MrV40 induces a TLR3-mediated inflammatory immune response in bone marrow-derived dendritic cells, indicating that a viral element likely contributes to the pathogenicity of MalasseZia.

Whole genome sequencing analysis of the cutaneous pathogenic yeast Malassezia restricta and identification of the major lipase expressed on the scalp of patients with dandruff

The genome of a clinical isolate of M. restricta was sequence and analysed to search for lipase orthologues and identify the lipase that is the most frequently expressed on the scalp of patients with dandruff, and it was found that the gene encoding lipase homolog MRES_03670, named LIP5 in this study, was the mostrequently expressed lipase in the swab samples.

Ambient pH regulates secretion of lipases in Malassezia furfur.

The qPCR results revealed that a set of functional lipase genes, LIP3-6, were constitutively expressed regardless of pH conditions or exposure time, concluding that the external pH plays a promotional role in the secretion of lipases but exerts less effect on transcription of the genes and morphology in M. furfur.

Fungal Screening on Olive Oil for Extracellular Triacylglycerol Lipases: Selection of a Trichoderma harzianum Strain and Genome Wide Search for the Genes

The success in identifying a lipase gene inducible with olive oil and the suitability of the functional screening and bioinformatic survey carried out herein, support the premise that the strategy can be used in other microorganisms with sequenced genomes to search for true lipases, or other enzymes belonging to large protein families.

Identification and Evaluation of Inhibitors of Lipase from Malassezia restricta using Virtual High-Throughput Screening and Molecular Dynamics Studies

A structure-based virtual screening in Zinc database to find the natural bioactive inhibitors of Mrlip1 and identify three compounds bearing superior affinity and specificity from the Traditional Chinese Medicine database that might act as promising lead drugs againstMrlip1.

Antifungal Mechanism of Action of Lauryl Betaine Against Skin-Associated Fungus Malassezia restricta

Lauryl betaine showed the most potent antifungal activity against Malassezia restricta and the mechanism of action was studied mainly using Cryptococcus neoformans, where the results indicated that the compound mainly affected cell membrane synthesis, particularly ergosterol synthesis.

The human fungal pathogen Malassezia and its role in cancer

A Novel Virus Alters Gene Expression and Vacuolar Morphology in <italic toggle='yes'>Malassezia</italic> Cells and Induces a TLR3-Mediated Inflammatory Immune Response

A novel virus alters gene expression and vacuolar morphology in Malassezia cells and induces a TLR3-mediated inflammatory immune response in bone marrow-derived dendritic cells, suggesting that a viral element contributes to the pathogenicity of MalasseZia.

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