Characterisation, genome size and genetic manipulation of the myxobacterium Sorangium cellulosum So ce56

@article{Pradella2002CharacterisationGS,
  title={Characterisation, genome size and genetic manipulation of the myxobacterium Sorangium cellulosum So ce56},
  author={Silke Pradella and Astrid Hans and Cathrin Spr{\"o}er and Hans Prof Dr Reichenbach and Klaus Gerth and Stefan Beyer},
  journal={Archives of Microbiology},
  year={2002},
  volume={178},
  pages={484-492}
}
Abstract. In this study, Sorangium cellulosum So ce56 was phenotypically and genotypically analysed in order to evaluate whether this strain can be used in a comprehensive genome project as a representative of the secondary metabolite-producing myxobacteria. In contrast to many other strains of S. cellulosum, strain So ce56 was found to have various advantageous features, including fast and homogeneous growth in submerged cultures and the ability to complete its morphological differentiation… Expand
Deciphering regulatory mechanisms for secondary metabolite production in the myxobacterium Sorangium cellulosum So ce56
TLDR
It is shown that transcription of the chivosazol biosynthetic genes (chiA–chiF) is directly controlled by this protein, and ChiR serves as a pleiotropic regulator in S. cellulosum, because mutant strains lack the ability to develop into regular fruiting bodies. Expand
19 Sorangium cellulosum
TLDR
The fascinating microorganisms of the genus Sorangium attract more and more attention, because they undergo a complex life cycle, possess the largest bacterial genomes known to date, and show a high potential as producers of biotechnologically important natural products. Expand
Complete genome sequence of the myxobacterium Sorangium cellulosum
TLDR
The complete genome sequence of the model Sorangium strain S. cellulosum So ce56 is reported, which produces several natural products and has morphological and physiological properties typical of the genus, and the circular genome is the largest bacterial genome sequenced to date. Expand
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Production of the Tubulin Destabilizer Disorazol in Sorangium cellulosum: Biosynthetic Machinery and Regulatory Genes
TLDR
The first electroporation protocol for a strain of the genus Sorangium is presented, and the transposon library was screened for disorazol‐negative mutants, leading to the identification of the corresponding trans‐acyltransferase core biosynthetic gene cluster. Expand
Identification and analysis of the chivosazol biosynthetic gene cluster from the myxobacterial model strain Sorangium cellulosum So ce56.
TLDR
The chivosazol biosynthetic gene locus belongs to the recently identified and rapidly growing class of trans-acyltransferase polyketide synthases, which do not contain acyltransferase domains integrated into the multimodular megasynthetases. Expand
Cloning, sequence analysis and disruption of the mglA gene involved in swarming motility of Sorangium cellulosum So ce26, a producer of the antifungal polyketide antibiotic soraphen A.
TLDR
The MglA protein, the product of the mglA gene, has been shown to be a central regulator of gliding motility and swarming in the related myxobacterium Myxococcus xanthus. Expand
30 Sorangium cellulosum Methods
TLDR
This chapter summarizes research methods to encourage further research into the complex biology of Sorangium cellulosum and uses the pMycoMarHyg transposon to construct developmental mutants of So ce56, and some of the resulting mutant phenotypes are shown in the chapter. Expand
Critical variations of conjugational DNA transfer into secondary metabolite multiproducing Sorangium cellulosum strains So ce12 and So ce56: development of a mariner-based transposon mutagenesis system.
TLDR
Using biparental and triparental mating, methodologies for DNA transfer from Escherichia coli via conjugation for the genome sequencing model strain So ce56 and the secondary metabolite multiproducing strain so ce12 are developed. Expand
NtcA: a negative regulator of secondary metabolite biosynthesis in Sorangium cellulosum.
TLDR
Results suggest that inhibition of chivosazol biosynthesis by environmental nitrogen is mediated, at least in part, by the NtcA protein, and reinforce the idea that genomics-guided engineering of regulatory pathways is a viable strategy for improving metabolite yields through fermentation. Expand
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