Chapter 11. Identification and analysis of tRNAs that are degraded in Saccharomyces cerevisiae due to lack of modifications.

@article{Chernyakov2008Chapter1I,
  title={Chapter 11. Identification and analysis of tRNAs that are degraded in Saccharomyces cerevisiae due to lack of modifications.},
  author={Irina Chernyakov and Melanie A Baker and Elizabeth J. Grayhack and Eric M Phizicky},
  journal={Methods in enzymology},
  year={2008},
  volume={449},
  pages={221-37}
}
Mounting evidence shows that tRNA modifications play crucial roles in the maintenance of wild-type levels of several tRNA species. This chapter describes a generalized framework in which to study tRNA turnover in the yeast Saccharomyces cerevisiae as a consequence of a defect in tRNA modification status. It describes several approaches for the identification of tRNA species that are reduced as a consequence of a modification defect, methods for analysis of the rate of tRNA loss and analysis of… CONTINUE READING

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