Chaperones-assisted soluble expression and maturation of recombinant Co-type nitrile hydratase in Escherichia coli to avoid the need for a low induction temperature.

Abstract

Nitrile hydratase (NHase) is an important industrial enzyme that biosynthesizes high-value amides. However, most of NHases expressed in Escherichia coli easily aggregate to inactive inclusion bodies unless the induction temperature is reduced to approximately 20°C. The NHase from Aurantimonas manganoxydans has been functionally expressed in E. coli, and… (More)
DOI: 10.1016/j.jbiotec.2015.03.004

Topics

Cite this paper

@article{Pei2015ChaperonesassistedSE, title={Chaperones-assisted soluble expression and maturation of recombinant Co-type nitrile hydratase in Escherichia coli to avoid the need for a low induction temperature.}, author={Xiaolin Pei and Qiuyan Wang and Lijun Meng and Jing Li and Zhengfen Yang and Xiaopu Yin and Lirong Yang and Shaoyun Chen and Jianping Wu}, journal={Journal of biotechnology}, year={2015}, volume={203}, pages={9-16} }