Chaperone-mediated activation in vivo of a Pseudomonas cepacia lipase

  title={Chaperone-mediated activation in vivo of a Pseudomonas cepacia lipase},
  author={Jesper L. Aamand and Audrey H. Hobson and Catherine M. F. Buckley and Steen T. J\orgensen and Borge Diderichsen and D. J. McConnell},
  journal={Molecular and General Genetics MGG},
An extracellular Pseudomonas cepacia lipase, LipA, is inactive when expressed in the absence of the product of the limA gene. Evidence has been presented that LimA is a molecular chaperone. The lipA and limA genes have been cloned in separate and independently inducible expression systems in Escherichia coli. These systems were used to test the molecular chaperone hypothesis by investigating whether LimA could activate presynthesized prelipase and whether presynthesized LimA could activate… CONTINUE READING

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Proceedings of the National Academy of Sciences of the United States of America • 1993
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