Chaperone–substrate interactions monitored via a robust TEM-1 β-lactamase fragment complementation assay

To investigate the application of the TEM-1 β-lactamase protein fragment complementation assay (PCA) in detecting weak and unstable protein–protein interactions as typically observed during chaperone-assisted protein folding in the periplasm of Escherichia coli. The TEM-1 β-lactamase PCA system effectively captured the interactions of three pairs of… CONTINUE READING