Channeling of substrates and intermediates in enzyme-catalyzed reactions.

@article{Huang2001ChannelingOS,
  title={Channeling of substrates and intermediates in enzyme-catalyzed reactions.},
  author={X. Huang and Hazel M. Holden and Frank M Raushel},
  journal={Annual review of biochemistry},
  year={2001},
  volume={70},
  pages={
          149-80
        }
}
The three-dimensional structures of tryptophan synthase, carbamoyl phosphate synthetase, glutamine phosphoribosylpyrophosphate amidotransferase, and asparagine synthetase have revealed the relative locations of multiple active sites within these proteins. In all of these polyfunctional enzymes, a product formed from the catalytic reaction at one active site is a substrate for an enzymatic reaction at a distal active site. Reaction intermediates are translocated from one active site to the next… Expand
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Direct Evidence for Metabolon Formation and Substrate Channeling in Recombinant TCA Cycle Enzymes.
TLDR
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Carbamoyl-phosphate Synthetase
TLDR
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  • Xinyi Huang, F. Raushel
  • Chemistry, Medicine
  • The Journal of Biological Chemistry
  • 2000
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TLDR
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Ammonia can replace glutamine as the ultimate source for the nitrogen requirement in the reaction presented above.3 A chemical mechanism for the enzymatic transformation is presented below in SchemeExpand
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TLDR
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TLDR
The change in reaction energetics of the bicarbonate-dependent ATPase reaction of Escherichia coli carbamoyl phosphate synthetase has been investigated for two site-directed mutations of the essential cysteine in the small subunit and results are consistent with the conclusion that the ground state for the enzyme-carboxyphosphate-ADP complex in the mutants is destabilized relative to the samecomplex in the wild type enzyme. Expand
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