Channel blocking drugs as tools to study glutamate receptors in insect muscles and molluscan neurons

  title={Channel blocking drugs as tools to study glutamate receptors in insect muscles and molluscan neurons},
  author={Marina V. Samoilova and E. V. Frolova and N. N. Potapjeva and Irina M. Fedorova and Valery E. Gmiro and Lev G Magazanik},
  journal={Invertebrate Neuroscience},
The action of three dicationic drugs, derivatives of adamantane (IEM-1460 and IEM-1754) and phencyclidine (IEM-1925), on glutamate receptors (GluRs) at the insect neuromuscular junction (Calliphora vicina larva) and on neurons of the freshwater gastropodian molluscPlanorbarius corneus has been studied using the voltage clamp technique. In the presence of concanavalin A complex glutamate-induced currents recorded from molluscan neurons reflected mainly the opening of cationic channels as a… 

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Characterization of ionotropic glutamate receptors in insect neuro-muscular junction.
Ion channels of glutamate receptors of nerve-muscle junction of larva of the fly Calliphora vicina demonstrate a high structural homology with vertebrate AMPA-channels
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Comparison of the Anticonvulsive Activities of Organic Mono- and Dications with Their Abilities to Inhibit NMDA and AMPA Glutamate Receptors
The anticonvulsive activity of I ÉM-1754 and IÉM-1925 were comparable with those of the known NMDA receptor blockers memantine and MK-801, and the complete correspondence between the in vitro data and in vivo results seen with some of the study compounds is evidently associated with their pharmacokinetic properties.
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Blockade of ion channels as an approach to studying AMPA receptor subtypes
  • L. Magazanik
  • Biology
    Neuroscience and Behavioral Physiology
  • 2006
Data on the possibility of selective pharmacological actions on the ion channels of different AMPA receptor subtypes are presented, which allows these receptors to be identified and their functions to be studied in greater depth in normal and pathological conditions.
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Novel adamantane derivatives act as blockers of open ligand-gated channels and as anticonvulsants.
Four novel adamantane derivatives appear to act on nicotinic ACh and NMDA receptors via similar mechanisms, although the voltage dependence of block suggested that the drugs bind at a more superficial site in the ACh-activated channel.
Quisqualate and ACPD are agonists for a glutamate-activated current in identified Aplysia neurons.
The results indicate that the glutamate receptor mediating the outward potassium response does not conform in its pharmacological profile to any of the known vertebrate glutamate receptor types.
Activation of a common potassium channel in molluscan neurones by glutamate, dopamine and muscarinic agonist.
Strong evidence was obtained against the involvement of any known secondary messenger systems (formation of nucleotides, phosphoinositide turnover and subsequent activation of protein kinase C, formation of nitric oxide, metabolism of arachidonic acid) in the transduction mechanism of F‐2268‐, dopamine‐ and glutamate‐induced responses.
Structure and Pharmacological Properties of a Molluscan Glutamate-Gated Cation Channel and its Likely Role in Feeding Behavior
The pharmacological properties and deduced location of Lym-eGluR2 are entirely consistent with it being (a component of) the receptor that mediates the excitatory effects of glutamate released from neurons within the feeding central pattern generator.
Different types of glutamate receptors in isolated and identified neurones of the mollusc Planorbarius corneus.
The data indicate that in the molluscan neurones studied there are at least three pharmacologically distinct glutamate receptors: (1) a receptor of quisqualate‐ibotenate type which directly controls chloride channel; (2) quisQualate and (3) kainate receptors which control in calcium‐independent manner the common potassium channel by activation of GTP‐binding protein.
Transduction mechanism for glutamate‐induced potassium current in neurones of the mollusc Planorbarius corneus.
The failure to demonstrate the involvement of any known secondary messenger systems in glutamate response transduction favours two assumptions: (1) the receptor‐G protein complex controls the potassium channel directly; or (2) some still unknown transduction system is used.
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