Changing the Regioselectivity of a P450 from C15 to C11 Hydroxylation of Progesterone

@article{Nguyen2012ChangingTR,
  title={Changing the Regioselectivity of a P450 from C15 to C11 Hydroxylation of Progesterone},
  author={Kim Thoa Nguyen and Cornelia Virus and Nils G{\"u}nnewich and Frank Hannemann and Rita Bernhardt},
  journal={ChemBioChem},
  year={2012},
  volume={13}
}
CYP106A2 is known as a 15β‐hydroxylase, but also shows minor 11α‐hydroxylase activity for progesterone. 11α‐Hydroxyprogesterone is an important pharmaceutical compound with anti‐androgenic and blood‐pressure‐regulating activity. This work therefore focused on directing the regioselectivity of the enzyme towards hydroxylation at position 11 in the C ring of the steroid through a combination of saturation mutagenesis and rational site‐directed mutagenesis. With the aid of data from a homology… Expand
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