Changes to the galactose/mannose ratio in galactomannans during coffee bean (Coffea arabica L.) development: implications for in vivo modification of galactomannan synthesis

  title={Changes to the galactose/mannose ratio in galactomannans during coffee bean (Coffea arabica L.) development: implications for in vivo modification of galactomannan synthesis},
  author={Robert J. Redgwell and Delphine Curti and John Rogers and Pierre Nicolas and Monica Fischer},
Endosperm was isolated from Arabica Caturra coffee beans 11, 15, 21, 26, 31 and 37 weeks after flowering, and the chemical composition and relative solubility of its component polysaccharides determined at each growth stage. Chemical analysis of the total mannan content of the cell wall material was done after solubilisation of galactomannans by alkaline extraction of the cell wall material followed by enzymatic digestion of the alkali-insoluble residue with a mixture of endo-mannanse and endo… Expand
Galactomannan content and key enzymes of its metabolism in seedsof cluster bean [Cyamopsis tetragonoloba (L.) Taub.]
It can be said that the s-D-mannosidase requires prior activity of s-1, 4-mannanase for galactomannan catabolism while a-galactosyltransferase activity is positively correlated with galactOMannan content and play a major role in guar gum synthesis and can be further used for gum improvement via genetic manipulation. Expand
Regulation of galactomannan biosynthesis in coffee seeds.
A microarray-based analysis of the developing seed transcriptome revealed that all genes of the core galactomannan synthesis machinery grouped in a single cluster of 209 co-expressed genes, suggesting a role for sorbitol and raffinose family oligosaccharides as transient auxiliary sources of building blocks for galactosyl side chains in coffee. Expand
Comparison and characterization of galactomannan at different developmental stages of Gleditsia sinensis Lam.
Rheological properties showed that apparent viscosity was largely influenced by the molecular weight and M/G ratio of galactomannans, which might result from primary galactOMannan biosynthesis and from galactose removal by α-galactosidase in the endosperm. Expand
Biochemical and molecular characterization of α-d-galactosidase from coffee beans
It is shown that α -galactosidase activities in field-grown coffee beans are variable amongst cultivars of the two species investigated ( Coffea arabica and C. canephora var. Robusta), and Southern-blot analysis suggests that the enzyme was encoded by at least two genes in C. arabicas that could explain the existence of theTwo isoforms identified in 2-DE profiles. Expand
A comprehensive review on leguminous galactomannans: structural analysis, functional properties, biosynthesis process and industrial applications.
This is the first comprehensive coverage on galactomannans which combines detailed structural and physicochemical properties as well as biology associated with the metabolism of galactosyl side chain, which focuses on different leguminous sources leading to various food and non-food applications of galactic biopolymers. Expand
Galactomannans in Coffee
Abstract Galactomannans are the most abundant constituents of coffee beans. Generically, the structure of these polysaccharides consists of a linear backbone of β-(1→4)-linked d -mannose residues,Expand
Variations in cell wall monosaccharide composition during seed development in Coffea arabica L. Comparison between Coffea arabica var. Bourbon and Coffea arabica var. Laurina
AbstractKey messageCell wall polysaccharide composition changed over seed development. Differences between B and BP only concerned the fruit growth period.AbstractCoffea arabica var. Laurina, alsoExpand
Cytochemistry and immunolocalisation of polysaccharides and proteoglycans in the endosperm of green Arabica coffee beans
It is demonstrated that the mannans and arabinogalactan proteins were located continuously across the width of the cell wall, but that the concentration of different structural epitopes within these polysaccharide types showed considerable spatial variation. Expand
Extraction, characterization and rheological behavior of galactomannans in high salinity and temperature conditions
Abstract Galactomannans can be extracted from various plant species, including those of the Cassia grandis Linn. f. (Fam. Caesalpiniaceae), a native Brazilian tree. The objective of this work was toExpand
Characterization and biotechnological application of an acid alpha-galactosidase from Tachigali multijuga Benth. seeds.
The alpha-galactosidase purified from germinating seeds had an M(r) of 38,000 and maximal activity at pH 5.0-5.5 and 50 degrees C, and in addition to its ability to hydrolyze raffinose and stachyose, the enzyme also hydrolyzed galactomannan. Expand


Control of mannose/galactose ratio during galactomannan formation in developing legume seeds
The endosperm α-galactosidase activity in Senna was capable, in vitro, of removing galactose from guar galactomannan without prior depolymerisation of the molecule, indicating a cause-and-effect relationship. Expand
Coffee bean arabinogalactans: acidic polymers covalently linked to protein.
An investigation of the structural features of the major AG fraction, released following enzymatic hydrolysis of the mannan-cellulose polymers, allowed a partial structure of coffee arabinogalactan to be proposed. Expand
In vivo modification of the cell wall polysaccharide galactomannan of guar transformed with a α-galactosidase gene cloned from senna
It is suggested that α-galactosidases can be involved in the determination of the final galactose content of endosperm galactomannans, demonstrating that cell wall polysaccharide biosynthesis can be modified in vivo. Expand
Polysaccharides of green Arabica and Robusta coffee beans.
It is concluded that there was no detectable difference between the Arabica and Robusta varieties of this study in their absolute polysaccharide content or in the gross structural features of their galactomannans. Expand
Effect of galactose content on the solution and interaction properties of guar and carob galactomannans
Abstract Guar galactomannan has been modified by treatment with an α- d -galactosidase A preparation from lucerne seeds. This enzyme was purified by affinity chromatography on N -ϵ-aminocaproyl-α- dExpand
Changes in Esterification of the Uronic Acid Groups of Cell Wall Polysaccharides during Elongation of Maize Coleoptiles.
The results indicate that the galactosyluronic acid units of grass pectic polysaccharides may be converted to other kinds of esters or form ester-like chemical interactions during expansion of the cell wall. Expand
Changes to the content of sugars, sugar alcohols, myo-inositol, carboxylic acids and inorganic anions in developing grains from different varieties of Robusta (Coffea canephora) and Arabica (C. arabica) coffees
Changes in concentration of mono- and oligosaccharides, sugar alcohols, myo-inositol, carboxylic acids and inorganic anions in coffee grains were analysed during grain development in three cultivarsExpand
Action patterns and substrate-binding requirements of β-D-mannanase with mannosaccharides and mannan-type polysaccharides
Abstract Purified (1→4)-β- d -mannanase from Aspergillus niger and lucerne seeds has been incubated with mannosaccharides and end-reduced (1→4)-β- d -mannosaccharides and, from the products ofExpand
Colorimetric Method for Determination of Sugars and Related Substances
Simple sugars, oligosaccharides, polysaccharides, and their derivatives, including the methyl ethers with free or potentially free reducing groups, give an orangeyellow color w-hen treated withExpand
A simple and rapid method for the permethylation of carbohydrates
Abstract As a result of a study of the permethylation of sugars in such dipolar aprotic solvents as methyl sulphoxide, the hitherto accepted role of the CH3SOCH−2 anion is questioned. The HO− and H−Expand