Changes to the galactose/mannose ratio in galactomannans during coffee bean (Coffea arabica L.) development: implications for in vivo modification of galactomannan synthesis

  title={Changes to the galactose/mannose ratio in galactomannans during coffee bean (Coffea arabica L.) development: implications for in vivo modification of galactomannan synthesis},
  author={Robert J. Redgwell and Delphine Curti and John Rogers and Pierre Nicolas and Monica Fischer},
Endosperm was isolated from Arabica Caturra coffee beans 11, 15, 21, 26, 31 and 37 weeks after flowering, and the chemical composition and relative solubility of its component polysaccharides determined at each growth stage. Chemical analysis of the total mannan content of the cell wall material was done after solubilisation of galactomannans by alkaline extraction of the cell wall material followed by enzymatic digestion of the alkali-insoluble residue with a mixture of endo-mannanse and endo… 

Galactomannan content and key enzymes of its metabolism in seedsof cluster bean [Cyamopsis tetragonoloba (L.) Taub.]

It can be said that the s-D-mannosidase requires prior activity of s-1, 4-mannanase for galactomannan catabolism while a-galactosyltransferase activity is positively correlated with galactOMannan content and play a major role in guar gum synthesis and can be further used for gum improvement via genetic manipulation.

Regulation of galactomannan biosynthesis in coffee seeds.

A microarray-based analysis of the developing seed transcriptome revealed that all genes of the core galactomannan synthesis machinery grouped in a single cluster of 209 co-expressed genes, suggesting a role for sorbitol and raffinose family oligosaccharides as transient auxiliary sources of building blocks for galactosyl side chains in coffee.

A comprehensive review on leguminous galactomannans: structural analysis, functional properties, biosynthesis process and industrial applications

This is the first comprehensive coverage on galactomannans which combines detailed structural and physicochemical properties as well as biology associated with the metabolism of galactosyl side chain, which focuses on different leguminous sources leading to various food and non-food applications of galactic biopolymers.

Variations in cell wall monosaccharide composition during seed development in Coffea arabica L. Comparison between Coffea arabica var. Bourbon and Coffea arabica var. Laurina

AbstractKey messageCell wall polysaccharide composition changed over seed development. Differences between B and BP only concerned the fruit growth period.AbstractCoffea arabica var. Laurina, also

Cytochemistry and immunolocalisation of polysaccharides and proteoglycans in the endosperm of green Arabica coffee beans

It is demonstrated that the mannans and arabinogalactan proteins were located continuously across the width of the cell wall, but that the concentration of different structural epitopes within these polysaccharide types showed considerable spatial variation.

Extraction, characterization and rheological behavior of galactomannans in high salinity and temperature conditions

Abstract Galactomannans can be extracted from various plant species, including those of the Cassia grandis Linn. f. (Fam. Caesalpiniaceae), a native Brazilian tree. The objective of this work was to



Control of mannose/galactose ratio during galactomannan formation in developing legume seeds

The endosperm α-galactosidase activity in Senna was capable, in vitro, of removing galactose from guar galactomannan without prior depolymerisation of the molecule, indicating a cause-and-effect relationship.

Coffee bean arabinogalactans: acidic polymers covalently linked to protein.

In vivo modification of the cell wall polysaccharide galactomannan of guar transformed with a α-galactosidase gene cloned from senna

It is suggested that α-galactosidases can be involved in the determination of the final galactose content of endosperm galactomannans, demonstrating that cell wall polysaccharide biosynthesis can be modified in vivo.

Polysaccharides of green Arabica and Robusta coffee beans.

Changes in Esterification of the Uronic Acid Groups of Cell Wall Polysaccharides during Elongation of Maize Coleoptiles.

The results indicate that the galactosyluronic acid units of grass pectic polysaccharides may be converted to other kinds of esters or form ester-like chemical interactions during expansion of the cell wall.

Colorimetric Method for Determination of Sugars and Related Substances

Simple sugars, oligosaccharides, polysaccharides, and their derivatives, including the methyl ethers with free or potentially free reducing groups, give an orangeyellow color w-hen treated with