Changes of low-frequency vibrational modes induced by universal 15N- and 13C-isotope labeling in S2/S1 FTIR difference spectrum of oxygen-evolving complex.

  title={Changes of low-frequency vibrational modes induced by universal 15N- and 13C-isotope labeling in S2/S1 FTIR difference spectrum of oxygen-evolving complex.},
  author={Y. Kimura and N. Mizusawa and A. Ishii and T. Yamanari and T. Ono},
  volume={42 45},
The effects of universal (15)N- and (13)C-isotope labeling on the low- (650-350 cm(-1)) and mid-frequency (1800-1200 cm(-1)) S(2)/S(1) Fourier transform infrared (FTIR) difference spectrum of the photosynthetic oxygen-evolving complex (OEC) were investigated in histidine-tagged photosystem (PS) II core particles from Synechocystis sp. PCC 6803. In the mid-frequency region, the amide II modes were predominantly affected by (15)N-labeling, whereas, in addition to the amide II, the amide I and… Expand
Ammonia-induced structural changes of the oxygen-evolving complex in photosystem II as revealed by light-induced FTIR difference spectroscopy.
The results showed that the effects of amines on the S(2)Q (A)(-)/S(1)Q(A) spectrum of PSII are sterically selective for small amines, and the correlations between the conditions and the conditions that give rise to the NH(3) induced significant structural changes of the OEC in the S (2) state are correlations based on the temperature of the FTIR measurement. Expand
Studies on photosynthetic oxygen-evolving complex by means of Fourier transform infrared spectroscopy: calcium and chloride cofactors
Structural roles of functional Ca2+ and Cl− ions in photosynthetic oxygen-evolving complexes (OEC) were studied using low- and mid- frequency S2/S1 Fourier transform infrared (FTIR) difference spectroscopy to enable the detection of subtle spectral changes. Expand
Evidence from FTIR difference spectroscopy of an extensive network of hydrogen bonds near the oxygen-evolving Mn(4)Ca cluster of photosystem II involving D1-Glu65, D2-Glu312, and D1-Glu329.
Analyses of the refined X-ray crystallographic structures of photosystem II have revealed the presence of possible channels for the removal of protons from the catalytic Mn(4)Ca cluster during the water-splitting reaction, and a negative band at 1747 cm(-1) was probed. Expand
Evidence that the C-terminus of the D1 polypeptide of photosystem II is ligated to the manganese ion that undergoes oxidation during the S1 to S2 transition: an isotope-edited FTIR study.
These experiments showed that tyrosine Y(D) may be structurally coupled to the carbonyl oxygen of an alanine-derived peptidecarbonyl group as well as to the wild-type and mutant PSII particles. Expand
Structural Changes of D1 C-terminal α-Carboxylate during S-state Cycling in Photosynthetic Oxygen Evolution*
The results indicate that the α-carboxylate of C-terminal Ala-344 is structurally associated with a manganese ion that becomes oxidized upon the S1-to-S2 transition and reduced reversely upon theS3- to-S0 transition but is not associated with manganESE ion(s) oxidized during the S0-to theS1 (and S2-to -S3) transition(s). Expand
FTIR detection of water reactions in the oxygen-evolving centre of photosystem II
  • T. Noguchi
  • Chemistry, Medicine
  • Philosophical Transactions of the Royal Society B: Biological Sciences
  • 2007
A possible mechanism of water oxidation to explain the FTIR data is proposed, while relatively high susceptibility to dehydration in the S2→S3 and S3→S0 transitions suggested the insertion of substrate water into the system during these transitions. Expand
Electrochemically induced FTIR difference spectroscopy in the mid- to far infrared (200 microm) domain: a new setup for the analysis of metal-ligand interactions in redox proteins.
It is shown that reliable reduced-minus-oxidized FTIR difference spectra are obtained, which correspond to single molecular vibrations, which are promising to analyze the infrared spectral region where metal-ligand vibrations are expected to contribute and to extend the analysis of vibrational properties to metal sites or redox states not accessible to (resonance) Raman spectroscopy. Expand
Evidence from FTIR difference spectroscopy that D1-Asp61 influences the water reactions of the oxygen-evolving Mn4CaO5 cluster of photosystem II.
  • R. Debus
  • Chemistry, Medicine
  • Biochemistry
  • 2014
This study characterizes the FTIR properties of the D1-D61A mutant of the cyanobacterium, Synechocystis sp. Expand
ATR-FTIR detection of metal-sensitive structural changes in the light-harvesting 1 reaction center complex from the thermophilic purple sulfur bacterium Thermochromatium tepidum.
Perfusion-induced attenuated total reflection (ATR) Fourier transform infrared (FTIR) spectroscopy is applied to highly purified LH1-RC complexes from Tch. Expand
Time-Resolved Single Frequency Infrared Absorption Spectroscopy on Photosystem II
In spite of recent advancements in structure elucidation of Photosystem II (PSII), by far not the complete catalytic cycle of photosynthetic water oxidation is sufficiently well understood. OpenExpand