Changes of RNA virus infection rates and gut microbiota in young worker Apis mellifera (Hymenoptera: Apidae) of a chalkbrood-infected colony after a pollination task in a greenhouse

  title={Changes of RNA virus infection rates and gut microbiota in young worker Apis mellifera (Hymenoptera: Apidae) of a chalkbrood-infected colony after a pollination task in a greenhouse},
  author={Hitoshi Matsumoto and Sachiyo Nomura and Yoichi Hayakawa},
  journal={Applied Entomology and Zoology},
Western honey bee (Apis mellifera Linnaeus) populations recently have been in decline worldwide owing not only to colony collapse disorder but also other infectious diseases. The problem is neither decreasing nor has it been resolved. Chalkbrood (Ascosphaera apis, Maassen ex Claussen) is a well-known fungal brood disease that is now found throughout the world, and there are indications that the incidence of chalkbrood may be on the rise. Here, we conducted comparative studies to analyze… 
4 Citations

Figures from this paper

Changes in Antioxidant Enzymes Activity and Metabolomic Profiles in the Guts of Honey Bee (Apis mellifera) Larvae Infected with Ascosphaera apis
Results suggest that A. apis infection may compromise the ability of infected larvae to cope with oxidative stress, providing new insight into changing patterns of physiological responses to A. Apis infection by concurrent use of conventional biochemical assays and untargeted metabolomics.
Chalkbrood: epidemiological perspectives from the host-parasite relationship.
  • S. Evison
  • Biology, Medicine
    Current opinion in insect science
  • 2015
To understand how to protect pollinators most effectively, it is crucial that future research takes a more ecologically relevant approach by studying the basic biology of the host-parasite relationship in the context of the multi-factorial processes that influence it.
Avian leukosis virus subgroup J infection influences the gut microbiota composition in chicken
The study indicates that ALV-J infection significantly altered the gut microbiota distribution in chickens and significantly influenced the abundance of L. helveticus and L. reuteri in the chicken gut.
An ecological approach to understanding gut microbiota and macrobiota interactions
An ecological approach is uses an ecological approach to understand microbiota-macrobiota interactions in a wild rodent system and highlights the importance of considering systemwide implications of a treatment or perturbation, particularly on gut microbiota- Macrobota interactions.


RT-PCR analysis of Deformed wing virus in honeybees (Apis mellifera) and mites (Varroa destructor).
Using a recently developed RT-PCR protocol for the detection of DWV, individual bees and mites originating from hives differing in Varroa infestation levels and the occurrence of crippled bees were analysed and virus replication in mites was correlated with wing deformity.
The transmission of deformed wing virus between honeybees (Apis mellifera L.) by the ectoparasitic mite varroa jacobsoni Oud
A positive relationship was found between increasing numbers of mites on individual bees and the incidence of morphological deformity and death and that much of the pathology previously associated with the effects of mite feeding could be attributed directly to secondary pathogens vectored by V. jacobsoni.
Changes in transcript abundance relating to colony collapse disorder in honey bees (Apis mellifera)
Ribosomal fragment abundance and presence of multiple viruses may prove to be useful diagnostic markers for colonies afflicted with CCD.
The prevalence of pathogens in honey bee (Apis mellifera) colonies infested with the parasitic mite Varroa jacobsoni
The evidence suggests that V. jacobsoni activates APV replication in adult bees by its feeding behaviour and transmits virus from adult honey bees to pupae, and adult bees, in which APV is multiplying, transmit the virus to unsealed brood in the larval food.
Microbial Ecology of the Hive and Pollination Landscape: Bacterial Associates from Floral Nectar, the Alimentary Tract and Stored Food of Honey Bees (Apis mellifera)
The results reveal that many bacteria prevalent in beebread and the crop are also found in floral nectar, suggesting frequent horizontal transmission, and do not support the core crop bacterial community hypothesized by recent studies.
The habitat disruption induces immune-suppression and oxidative stress in honey bees
It is found that the greenhouse environment changes the gene expression profiles and induces immune-suppression and oxidative stress in honey bees, and honey bee colonies are likely to collapse during pollination in greenhouses when heavily infested with pathogens.
Pathogen Webs in Collapsing Honey Bee Colonies
Although pathogen identities differed between the eastern and western United States, there was a greater incidence and abundance of pathogens in CCD colonies, and novel strains of the recently described Lake Sinai viruses were identified and found evidence of a shift in gut bacterial composition that may be a biomarker of CCD.
A simple and distinctive microbiota associated with honey bees and bumble bees
It is found that most bee species lack phylotypes that are the same or similar to those typical of A. mellifera, rejecting the hypothesis that this dietary transition was symbiont‐dependent and potentially key to the maintenance of a more consistent gut microbiota.
Diversity and phylotype consistency of bacteria in the guts of three bee species (Apoidea) at an oilseed rape field.
The gut of insects may harbour one of the largest reservoirs of a yet unexplored microbial diversity, and phylogenetic analyses indicated common bacterial phylotypes for all three bee species, e.g. those related to Simonsiella, Serratia, and Lactobacillus.
Functional diversity within the simple gut microbiota of the honey bee
The sequenced metagenome of the gut microbiota of honey bees reveals insights into mutualistic functions governed by the microbiota of this important pollinator and indicates that the honey bee can serve as a model for understanding more complex gut-associated microbial communities.