Changes of DNA methylation and hydroxymethylation in plant protoplast cultures.

@article{Moricov2013ChangesOD,
  title={Changes of DNA methylation and hydroxymethylation in plant protoplast cultures.},
  author={Pavla Moricov{\'a} and Vladan Ondřej and Bo{\vz}ena Navr{\'a}tilov{\'a} and Lenka Luhov{\'a}},
  journal={Acta biochimica Polonica},
  year={2013},
  volume={60 1},
  pages={
          33-6
        }
}
Cytosine methylation patterns in higher eukaryotes are important in gene regulation. Along with 5-methylcytosine (5-mC), a newly discovered constituent of mammalian DNA, 5-hydroxymethylcytosine (5-hmC), is the other modified base in higher organisms. In this study we detected 5-hmC in plant protoplast DNA and demonstrated its increasing content during the first 72 hrs. of protoplast cultivation. In contrast to 5-hmC, the amount of 5-mC decreased during protoplast cultivation. It was also found… CONTINUE READING

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