Changes in viability and macromolecular content of long-term batch cultures of Salmonella typhimurium measured by flow cytometry.

@article{Turner2000ChangesIV,
  title={Changes in viability and macromolecular content of long-term batch cultures of Salmonella typhimurium measured by flow cytometry.},
  author={Kristy L Turner and J F Porter and Roger W. Pickup and Christine Edwards},
  journal={Journal of applied microbiology},
  year={2000},
  volume={89 1},
  pages={90-9}
}
Exposure of many Gram-negative bacteria to prolonged starvation induces alternative programmes of gene expression, along with a transition into a dormant condition sometimes referred to as a viable non-culturable (VBNC) state. Knowledge of how pathogenic species respond to nutrient limitation is therefore important for their detection and dissemination. This study used flow cytometry, coupled with fluorescent dyes for viability and macromolecular content, to study the responses of the pathogen… CONTINUE READING

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