Changes in the ratio of bioactive to immunoreactive adrenocorticotropin-like activity released by pituitary cells from ovine fetuses and lambs.

@article{Brieu1987ChangesIT,
  title={Changes in the ratio of bioactive to immunoreactive adrenocorticotropin-like activity released by pituitary cells from ovine fetuses and lambs.},
  author={V. Brieu and Philippe Durand},
  journal={Endocrinology},
  year={1987},
  volume={120 3},
  pages={936-40}
}
We have compared the biological (B) and immunological (I) ACTH-like activities (ALA) released by short term cultured pituitary cells from fetal (63-144 days old) and young lambs (7-150 days old) both under basal conditions, and when stimulated by ovine(o)CRF-(1-41) or arginine vasopressin (AVP), separately or in combination. The bioassay was based on the production of corticosteroids by cultured adrenal cells from adult sheep. The standard used in both the RIA and the bioassay was synthetic… CONTINUE READING
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