Changes in the organization of the actin cytoskeleton during preimplantation development of the pig embryo.

@article{Albertini1987ChangesIT,
  title={Changes in the organization of the actin cytoskeleton during preimplantation development of the pig embryo.},
  author={David F Albertini and Eric W Overstrom and Kristin M Ebert},
  journal={Biology of reproduction},
  year={1987},
  volume={37 2},
  pages={441-51}
}
The organization of the actin cytoskeleton was studied in unfertilized porcine oocytes and preimplantation stage embryos from Day 1 through Day 8 of development. Fixed and detergent-extracted oocytes and embryos were analyzed by fluorescence microscopy after staining with either rhodamine-phalloidin to localize filamentous actin or with affinity-purified anti-actin antibodies to localize the total immunodetectable actin. Whereas unfertilized oocytes contain immunoreactive cytoplasmic actin… CONTINUE READING

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