[Changes in the contents of membrane phospholipid and membrane phospholipid asymmetry in glucose-6-phosphate dehydrogenase deficient erythrocyte].

  • C Zou, X Tian, H Yan
  • Published 1998 in
    Zhonghua xue ye xue za zhi = Zhonghua xueyexue…

Abstract

OBJECTIVE To study the effect of G6PD deficiency on membrane phospholipid structures. METHODS Treatment of intact erythrocyte with phospholipase A2 was used for the membrane phospholipid asymmetry. Membrane phospholipid was determined by high performance liquid chromatography (HPLC). RESULTS The contents of phosphatidylethanolamine (PE), phosphatidylcholine (PC), phosphatidylserine (PS) were 5.18 +/- 1.86, 1.51 +/- 0.58 and 5.70 +/- 1.91 mg/mg membrane protein, respectively, in G6PD deficient erythrocytes and were 5.59 +/- 1.16, 2.92 +/- 0.50 and 6.64 +/- 0.92 mg/mg membrane protein, respectively, in normal erythrocytes. In normal erythrocytes, 17.6% +/- 2.5% PE and 57.9% +/- 4.7% PC were present and no PS was found on the outer leaflet of the membrane. While in G6PD deficient ones, 30.2% +/- 5.7% PE, 53.8% +/- 7.4% PC and 27.2% +/- 5.8% PS were present on the outer leaflet. CONCLUSION Membrane phospholipid changes in G6PD deficient erythrocytes might be one of the factors causing hemolysis.

Cite this paper

@article{Zou1998ChangesIT, title={[Changes in the contents of membrane phospholipid and membrane phospholipid asymmetry in glucose-6-phosphate dehydrogenase deficient erythrocyte].}, author={C Zou and X Tian and H Yan}, journal={Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi}, year={1998}, volume={19 4}, pages={192-4} }