Changes in the concentration and fatty acid composition of phosphoinositides induced by hormones in hepatocytes.

@article{Augert1989ChangesIT,
  title={Changes in the concentration and fatty acid composition of phosphoinositides induced by hormones in hepatocytes.},
  author={Guy Augert and Peter F. Blackmore and John H. Exton},
  journal={The Journal of biological chemistry},
  year={1989},
  volume={264 5},
  pages={2574-80}
}
The hormonal regulation of phosphoinositide levels in isolated hepatocytes was studied using chemical means. Extracted inositol phospholipids were adsorbed to neomycin-coated glass beads and then eluted and quantitated by charring after separation by thin layer chromatography on silica gel. The amounts (in nanograms/mg wet weight) of phosphatidylinositol 4,5-bisphosphate (PIP2), phosphatidylinositol 4-phosphate (PIP), and phosphatidylinositol (PI) were 20 +/- 1, 16 +/- 1, and 1790 +/- 140… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 14 extracted citations

Molecular Mechanisms of Cellular Growth

Developments in Molecular and Cellular Biochemistry • 1991
View 10 Excerpts
Highly Influenced

Similar Papers

Loading similar papers…