Changes in properties of phosphoenolpyruvate carboxylase from the CAM plant Sedum praealtum D.C. upon dark/light transition and their stabilization by glycerol

@article{Manetas1982ChangesIP,
  title={Changes in properties of phosphoenolpyruvate carboxylase from the CAM plant Sedum praealtum D.C. upon dark/light transition and their stabilization by glycerol},
  author={Yiannis Manetas},
  journal={Photosynthesis Research},
  year={1982},
  volume={3},
  pages={321-333}
}
A prenounced decrease in phosphoenolpyruvate earboxylase (PEPC) activity is observed upon dark/light transition in Sedum praealtum D.C., only when glycerol is included in the extraction medium. If glycerol is omitted, the activity extracted in light is initially low, but soon reaches night levels. The stabilization of the light-induced form of the enzyme by glycerol, in crude or desalted extracts, made it possible to study its kinetic properties in comparison to those of the dark form. The… CONTINUE READING