Changes in order parameters associated with ceramide-mediated membrane reorganization measured using pTIRFM.

@article{Ramirez2013ChangesIO,
  title={Changes in order parameters associated with ceramide-mediated membrane reorganization measured using pTIRFM.},
  author={Daniel M. Carter Ramirez and Zygmunt J. Jakubek and Zhengfang Lu and William W. Ottawa Ogilvie and Linda J Johnston},
  journal={Langmuir : the ACS journal of surfaces and colloids},
  year={2013},
  volume={29 51},
  pages={
          15907-18
        }
}
The enzymatic generation of ceramide has significant effects on the biophysical properties of lipid bilayers and can lead to the extensive reorganization of cell membranes. We have synthesized and characterized a headgroup-labeled fluorescent lipid probe (NBD-ceramide, NBD-Cer) and demonstrated that it can be used for polarized total internal reflection fluorescence microscopy experiments to probe changes in membrane order that result from ceramide incorporation. NBD-Cer measures significantly… CONTINUE READING
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