Changes in internal pH associated with initiation of motility and acrosome reaction of sea urchin sperm.

@article{Lee1983ChangesII,
  title={Changes in internal pH associated with initiation of motility and acrosome reaction of sea urchin sperm.},
  author={Heui Lee and Carl Hirschie Johnson and David Epel},
  journal={Developmental biology},
  year={1983},
  volume={95 1},
  pages={
          31-45
        }
}
The changes in the intracellular pH (pHi) of sea urchin sperm associated with motility initiation and acrosome reaction were investigated using uptake of two different probes; 9-aminoacridine and methylamine, as a qualitative index. Sperm suspended in Na+-free sea water were immotile and able to concentrate these amines 20-fold or greater indicating that pHi is more acidic than the external medium (pHo = 7.7). This uptake ratio was essentially constant over a wide range of probe and sperm… CONTINUE READING
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