Changes in growth patterns and intracellular calcium concentrations in Aspergillus awamori treated with amphotericin B

Abstract

Growth patterns and intracellular Ca2+ concentrations in the mutant strain Aspergillus awamori 66A containing a recombinant aequorin gene were studied in the presence of a permeabilizing fungicidal agent amphotericin B. The cell response, i.e., changes in the growth and development of the fungus (initiation of spore germination, mycelial growth, and intensity of sporulation) was dose-dependent. Low concentrations of amphotericin B (2.5 μM) stimulated spore germination: the number of germinating spores was 2–3 times higher than in the control (without the fungicide). At higher amphotericin concentrations (20 μM) spore germination was inhibited. Amphotericin B had a dose-dependent effect on mycelial growth and sporulation intensity on solid Vogel medium. Intracellular Ca2+ concentrations in the presence of amphotericin B were investigated using the luminescence of the photoprotein aequorin. High concentrations of amphotericin B (10 and 20 μM) were shown to cause an instantaneous increase in Ca2+ concentrations compared to the control and lower amphotericin concentration (2.5 μM). Ca2+ concentrations remained elevated throughout the experiment and correlated with the inhibition of mycelial growth and development.

DOI: 10.1134/S002626171404002X

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@article{ElRahman2014ChangesIG, title={Changes in growth patterns and intracellular calcium concentrations in Aspergillus awamori treated with amphotericin B}, author={Abeer Abd El-Rahman and Olga Kozlova and Sally Mohamed Abd El-Aziz El-Shafei and Farida Kashifovna Alimova and F. G. Kupriyanova-Ashina}, journal={Microbiology}, year={2014}, volume={83}, pages={344-351} }