Changes in gene expression profiles in developing B cells of murine bone marrow.

@article{Hoffmann2002ChangesIG,
  title={Changes in gene expression profiles in developing B cells of murine bone marrow.},
  author={Reinhard Hoffmann and Thomas Seidl and Martin Neeb and Antonius G. Rolink and Fritz Georg Melchers},
  journal={Genome research},
  year={2002},
  volume={12 1},
  pages={98-111}
}
Gene expression profiles of five consecutive stages of mouse B cell development were generated with high-density oligonucleotide arrays from as few as 2 x 10(4) ex vivo isolated and flow-cytometrically purified cells. Between 2.8% and 6.8% of all genes change on differentiation from one cellular stage to the next by at least twofold. The entire pathway involves differential expression of 10.7% of all genes. Previously known expression patterns of 15 genes (like surrogate light chain, RAG-1/2… CONTINUE READING