Changes in expression of monoclonal antibody epitopes on laminin-5r induced by cell contact.

@article{Plopper1996ChangesIE,
  title={Changes in expression of monoclonal antibody epitopes on laminin-5r induced by cell contact.},
  author={G Plopper and J Falk-Marzillier and Stefan Glaser and M I Fitchmun and Gianluigi Giannelli and Tammy Romano and Jonathan C R Jones and Vito Quaranta},
  journal={Journal of cell science},
  year={1996},
  volume={109 ( Pt 7)},
  pages={1965-73}
}
Laminin-5r is a basement membrane component that promotes rapid adhesion and hemidesmosome formation in epithelial cells. We raised monoclonal antibodies and identified their corresponding epitopes on the constituent chains of laminin-5r by western blotting. Using a combination of immunoprecipitation and ELISA assays, we determined that these epitopes are differentially exposed on two forms of the laminin-5r heterotrimer: soluble (passively adsorbed onto plastic) and cell-associated. Antibody… CONTINUE READING
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