Changes in cinnamyl alcohol dehydrogenase activities from sugarcane cultivars inoculated with Sporisorium scitamineum sporidia.

@article{Santiago2012ChangesIC,
  title={Changes in cinnamyl alcohol dehydrogenase activities from sugarcane cultivars inoculated with Sporisorium scitamineum sporidia.},
  author={Roc{\'i}o Santiago and Borja Alarc{\'o}n and Roberto de Armas and Carlos Miguel Oliveira Vicente and Mar{\'i}a Estrella Legaz},
  journal={Physiologia plantarum},
  year={2012},
  volume={145 2},
  pages={245-59}
}
This study describes a method for determining cinnamyl alcohol dehydrogenase activity in sugarcane stems using reverse phase (RP) high-performance liquid chromatography to elucidate their possible lignin origin. Activity is assayed using the reverse mode, the oxidation of hydroxycinnamyl alcohols into hydroxycinnamyl aldehydes. Appearance of the reaction products, coniferaldehyde and sinapaldehyde is determined by measuring absorbance at 340 and 345 nm, respectively. Disappearance of substrates… CONTINUE READING