Changes in chromatin compaction during the cell cycle revealed by micrometer-scale measurement of molecular flow in the nucleus.

Abstract

We present a quantitative fluctuation-based assay to measure the degree of local chromatin compaction and investigate how chromatin density regulates the diffusive path adopted by an inert protein in dividing cells. The assay uses CHO-K1 cells coexpressing untagged enhanced green fluorescent protein (EGFP) and histone H2B tagged mCherry. We measure at the single-cell level the EGFP localization and molecular flow patterns characteristic of each stage of chromatin compaction from mitosis through interphase by means of pair-correlation analysis. We find that the naturally occurring changes in chromatin organization impart a regulation on the spatial distribution and temporal dynamics of EGFP within the nucleus. Combined with the analysis of Ca(2+) intracellular homeostasis during cell division, EGFP flow regulation can be interpreted as the result of controlled changes in chromatin compaction. For the first time, to our knowledge, we were able to probe chromatin compaction on the micrometer scale, where the regulation of molecular diffusion may become relevant for many cellular processes.

DOI: 10.1016/j.bpj.2011.11.4026
0100200201220132014201520162017
Citations per Year

222 Citations

Semantic Scholar estimates that this publication has 222 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Hinde2012ChangesIC, title={Changes in chromatin compaction during the cell cycle revealed by micrometer-scale measurement of molecular flow in the nucleus.}, author={Elizabeth Hinde and Francesco Cardarelli and Michelle A Digman and Enrico Gratton}, journal={Biophysical journal}, year={2012}, volume={102 3}, pages={691-7} }