Changes in Polysome Association of mRNA Throughout Growth and Development in Arabidopsis thaliana.

@article{Yamasaki2015ChangesIP,
  title={Changes in Polysome Association of mRNA Throughout Growth and Development in Arabidopsis thaliana.},
  author={Shotaro Yamasaki and Hideyuki Matsuura and Taku Demura and Ko Kato},
  journal={Plant \& cell physiology},
  year={2015},
  volume={56 11},
  pages={
          2169-80
        }
}
Translational control is a key regulatory step in the expression of genes as proteins. In plant cells, the translational efficiency of mRNAs differs for different mRNA species, and the efficiency dynamically changes in various conditions. To gain a global view of translational control throughout growth and development, we performed genome-wide analysis of polysome association of mRNA during growth and leaf development in Arabidopsis thaliana by subjecting the mRNAs in polysomes to DNA… 
View on PubMed
academic.oup.com
20 Citations
Background Citations
5
Methods Citations
2

Figures and Tables from this paper

20 Citations

Citation Type

Citation Type

Emerging Roles and Landscape of Translating mRNAs in Plants
TLDR
The processes affecting the abundance of translating mRNAs, their regulation during plant development and tolerance to stress conditions and plant factors affecting the selection of translating mRNA pools are examined.
Changes in mRNA degradation efficiencies under varying conditions are regulated by multiple determinants in Arabidopsis thaliana.
TLDR
It is suggested that several determinants govern the efficiency of mRNA degradation in plants, helping the organism to adapt to varying conditions by controlling mRNA accumulation.
Translation regulation in plants: an interesting past, an exciting present and a promising future.
TLDR
The use of high-throughput approaches to map and quantify different critical parameters affecting translation, such as RNA structure, protein-RNA interactions and ribosome occupancy at the genome level, holds the promise to decipher the likely complex and diverse, but also fascinating, mechanisms behind the regulation of translation.
Translational gene regulation in plants: A green new deal
TLDR
Important new insights into translational control on cytosolic ribosomes continue to emerge and sketches of coherent signaling pathways that connect external stimuli with a translational response are emerging.
Computational and Experimental Tools to Monitor the Changes in Translation Efficiency of Plant mRNA on a Genome-Wide Scale: Advantages, Limitations, and Solutions
TLDR
The aims of this review are to describe the experimental approaches for investigation of differential translation in plants on a genome-wide scale, summarize the current data on computational algorithms for detection of specific structure–function features and key determinants in plant mRNAs and their correlation with translation efficiency.
Arabidopsis thaliana cold-regulated 47 gene 5'-untranslated region enables stable high-level expression of transgenes.
Multiplexing polysome profiling experiments to study translation in Escherichia coli
TLDR
A multiplex polysome profiling experiment in which distinct cellular extracts are pooled before loading on the sucrose gradient for fractionation is proposed, useful to study the translational effect of mRNA sequence-dependent parameters that often require testing multiple samples and conditions in parallel.
Study of regulatory factors of translation in Escherichia coli
TLDR
It is shown that an increase in mRNA concentration by transcriptional induction results in increases in percentage of copies in translation and in ribosome load, and this effect of transcription on translation has been validated at the molecular level on several genes.
Differential Expression of Genes of the Calvin-Benson Cycle and its Related Genes During Leaf Development in Rice.
TLDR
Genes of the Calvin-Benson cycle and its related genes were differentially expressed during leaf development in rice, suggesting that such differential gene expression is necessary for formation and maintenance of the machinery of photosynthetic carbon assimilation.
BREAKTHROUGH REPORT - Large scale measurement of protein synthesis rates over the diurnal cycle reveal pathway specific regulation of translation
TLDR
Diurnal protein degradation rate was observed to be tightly coordinated with protein synthesis rate as few leaf proteins changed in abundance despite reduced translation rates during the night, revealing how the pausing of photosystem synthesis and degradation at night allows the redirection of a decreased energy budget to a selective night-time maintenance schedule.
...
1
2
...

References

SHOWING 1-10 OF 40 REFERENCES
mRNA sequence features that contribute to translational regulation in Arabidopsis
TLDR
DNA microarrays were used to evaluate the regulation of the proportion of individual mRNA species in polysomal complexes in leaves of Arabidopsis thaliana under control growth conditions and following a mild dehydration stress, indicating that additional factors contribute to translational regulation inArabidopsis.
Genome-wide analysis of transcript abundance and translation in Arabidopsis seedlings subjected to oxygen deprivation.
TLDR
Evaluation of quantitative features of mRNA sequences demonstrated that a low GC nucleotide content of the 5'-untranslated region provides a selective advantage for translation under hypoxia, contributing to the differential regulation of gene expression in response to oxygen deprivation.
Analysis of Genome-Wide Changes in the Translatome of Arabidopsis Seedlings Subjected to Heat Stress
TLDR
A genome-wide analysis is carried out to monitor the changes in the translation efficiency of individual mRNAs of Arabidopsis thaliana seedlings after the exposure to a heat shock stress, demonstrating that translation exerts a wide but dual regulation of gene expression.
Differential mRNA translation contributes to gene regulation under non-stress and dehydration stress conditions in Arabidopsis thaliana.
TLDR
The abundance of PS mRNA may provide a more accurate estimate of gene expression than total cellular mRNA because of extensive differential translational regulation.
Large-Scale Analysis of mRNA Translation States during Sucrose Starvation in Arabidopsis Cells Identifies Cell Proliferation and Chromatin Structure as Targets of Translational Control1[W]
TLDR
Starvation-induced translational control also affects chromatin regulation genes, such as the HD1 histone deacetylase, and the level of histone H4 acetylation was found to increase during starvation, suggesting that regulation of the global nuclear transcriptional activity might be linked to cytoplasmic translational regulations.
Translational Regulation of Cytoplasmic mRNAs
TLDR
This review summarizes the diversity of translational control mechanisms in the plant cytoplasm, focusing on specific cases where mechanisms of translatory control have evolved to complement or eclipse other levels of gene regulation.
Dynamic Light Regulation of Translation Status in Arabidopsis thaliana
TLDR
It is proposed that modulation of translation of a subset of cellular mRNAs functions as an energy conservation mechanism in response to changes in oxygen availability.
Widespread translational control contributes to the regulation of Arabidopsis photomorphogenesis
TLDR
A previously neglected aspect of gene expression regulation during Arabidopsis photomorphogenesis is reported, and it is revealed that mRNAs regulated at the translational level share characteristics of longer half‐lives and shorter cDNA length, and that transcripts with a cis‐element, TAGGGTTT, in their 5′ untranslated region have higher translatability.
Genome-wide analyses of early translational responses to elevated temperature and high salinity in Arabidopsis thaliana.
TLDR
The results highlight the importance of dynamic changes in mRNA translation, which include selective translation and extensive repression of a subset of transcripts, in plant abiotic stress responses.
Cell-type specific analysis of translating RNAs in developing flowers reveals new levels of control
TLDR
This study describes both the expression profiles of cells within spatiotemporal domains of the Arabidopsis thaliana flower and the post‐transcriptional regulation of these mRNAs, at nucleotide resolution, and identifies a new class of noncoding RNAs associated with polysomes.
...
1
2
3
4
...