• Corpus ID: 43194262

Changes in Michaelis and spectral constants for aniline in hepatic microsomes from phenobarbital-treated rats.

  title={Changes in Michaelis and spectral constants for aniline in hepatic microsomes from phenobarbital-treated rats.},
  author={Anthony M. Guarino and Theodore E. Gram and Philippe Gigon and Frank E. Greene and James R. Gillette},
  journal={Molecular pharmacology},
  volume={5 2},
Recent work has suggested that the rate of hepatic microsomal drug metabolism may be correlated with either the cytochrome P-450 content or the magnitude of the spectral change caused by the addition of substrates to microsomal suspensions. Accordingly, the relationships between these factors were investigated in 0.9% NaCl- and phenobarbital-treated intact, sham-operated, and partially hepatectomized rats. In intact animals phenobarbital produced a 3-4-fold increase in both microsomal aniline… 
Evidence for two catalytically different binding sites of liver microsomal cytochrome P-450: importance for species and sex differences in oxidation pattern of lidocaine.
The data suggest that the two spectral phases represent two binding sites of cytochrome P-450 each having a certain "catalytic specificity" - the high affinity catalyzing aromatic hydroxylation and the "low-affinity site" deethylation, which is supported by the observed differential effects of pH and MgCl2 concentration on the two types of oxidation.
Kinetic experiments on the binding of metyrapone to liver microsomes
It is concluded that the degree of inhibition is correlated to the amount of metyrapone bound to cytochrome P-450, and possible mechanisms for explaining competitive inhibition are discussed.
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Benzene metabolism in mouse liver microsomes.
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  • H. Raza, W. Montague
  • Biology, Chemistry
    Comparative biochemistry and physiology. C, Comparative pharmacology and toxicology
  • 1993
Dietary lipotropes, hepatic microsomal mixed-function oxidase activities, and in vivo covalent binding of aflatoxin B1 in rats.
The results suggest that the marginally lipotrope-deficient diet does not enhance tumor formation through an increased microsomal activation of AFB1, and the depression of DNA and RNA adduct formation in the Diet 2 animals was probably related to the lower mixed-function oxidase activities.