Changes in DNA methylation during somatic embryogenesis in Cucurbita pepo L.

@article{LeljakLevani2004ChangesID,
  title={Changes in DNA methylation during somatic embryogenesis in Cucurbita pepo L.},
  author={Dunja Leljak-Levani{\'c} and Nata{\vs}a Bauer and Snje{\vz}ana Mihaljevi{\'c} and Sibila Jelaska},
  journal={Plant Cell Reports},
  year={2004},
  volume={23},
  pages={120-127}
}
Three pumpkin embryogenic lines were initiated on wounded zygotic embryos cultured on medium with or without 2,4-dichlorophenoxyacetic acid (2,4-D). Somatic embryo development was controlled by the availability of various compounds in the medium: presence/absence of 2,4-D, nitrogen sources. The highest rate of DNA methylation was in the early embryo stages, predominantly on MSC medium with 2,4-D and on auxin-free medium supplemented with 1.0 mM NH4Cl. DNA methylation was correlated with early… CONTINUE READING