Challenges of CRISPR/Cas9 applications for long non-coding RNA genes

  title={Challenges of CRISPR/Cas9 applications for long non-coding RNA genes},
  author={Ashish Goyal and Ksenia Myacheva and Matthias Gross and Marcel Klingenberg and Berta Duran Arqu{\'e} and Sven Diederichs},
  booktitle={Nucleic acids research},
The CRISPR/Cas9 system provides a revolutionary genome editing tool for all areas of molecular biology. In long non-coding RNA (lncRNA) research, the Cas9 nuclease can delete lncRNA genes or introduce RNA-destabilizing elements into their locus. The nuclease-deficient dCas9 mutant retains its RNA-dependent DNA-binding activity and can modulate gene expression when fused to transcriptional repressor or activator domains. Here, we systematically analyze whether CRISPR approaches are suitable to… CONTINUE READING
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