Challenges in designing a Taqman-based multiplex assay for the simultaneous detection of Herpes simplex virus types 1 and 2 and Varicella-zoster virus.

@article{Weidmann2008ChallengesID,
  title={Challenges in designing a Taqman-based multiplex assay for the simultaneous detection of Herpes simplex virus types 1 and 2 and Varicella-zoster virus.},
  author={Manfred Weidmann and Katrin Armbruster and Frank T. Hufert},
  journal={Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology},
  year={2008},
  volume={42 4},
  pages={326-34}
}
To optimise molecular detection of herpesviruses an internally controlled multiplex Taqman-PCR for the detection of Herpes simplex virus 1 (HSV1), Herpes simplex virus 2 (HSV2) and Varicella-zoster virus (VZV) was developed. The selection of the dye combination working on the ABI 7700 cycler for this multiplex PCR revealed crosstalk phenomena between… CONTINUE READING