ChIP‐seq reveals a role for CrzA in the Aspergillus fumigatus high‐osmolarity glycerol response (HOG) signalling pathway

@article{Castro2014ChIPseqRA,
  title={ChIP‐seq reveals a role for CrzA in the Aspergillus fumigatus high‐osmolarity glycerol response (HOG) signalling pathway},
  author={Patr{\'i}cia A. Castro and Chenxi Chen and Ricardo S C Almeida and Fernanda Zanolli Freitas and Maria C{\'e}lia Bertolini and Enyara R Morais and Neil Andrew Brown and Leandra Naira Zambelli Ramalho and Daisuke Hagiwara and Thomas K. Mitchell and Gustavo Henrique Goldman},
  journal={Molecular Microbiology},
  year={2014},
  volume={94}
}
Aspergillus fumigatus is an opportunistic pathogen and allergen of mammals. Calcium signalling is essential for A. fumigatus pathogenicity and is regulated by the CrzA transcription factor. We used ChIP‐seq (Chromatin Immunoprecipitation DNA sequencing) to explore CrzA gene targets in A. fumigatus. In total, 165 potential binding peaks including 102 directly regulated genes were identified, resulting in the prediction of the A[GT][CG]CA[AC][AG] CrzA‐binding motif. The 102 CrzA putatively… 

The Aspergillus fumigatus Phosphoproteome Reveals Roles of High-Osmolarity Glycerol Mitogen-Activated Protein Kinases in Promoting Cell Wall Damage and Caspofungin Tolerance

This work investigates the global phosphoproteome of the ΔsakA and ΔmpkC A. fumigatus and high-osmolarity glycerol (HOG) pathway MAPK mutants upon cell wall damage to provide understanding of how fungal signal transduction networks modulate the cell wall.

Calcineurin‐dependent dephosphorylation of the transcription factor CrzA at specific sites controls conidiation, stress tolerance, and virulence of Aspergillus fumigatus

It is demonstrated that CrzA activity during calcium stimulation can only be suppressed by a high degree of phosphorylation in multiple regions of the protein, and the findings suggest that this regulation is not solely accomplished through control of CrZA nuclear import.

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The Aspergillus fumigatus CrzA Transcription Factor Activates Chitin Synthase Gene Expression during the Caspofungin Paradoxical Effect

The CPE is dependent on the cell wall integrity (CWI) mitogen-activated protein kinase MpkAMPK1 and its associated transcription factor (TF) RlmARLM1, which regulate chitin synthase gene expression in response to different concentrations of caspofungin, and shows that the regulation of cell wall biosynthetic genes occurs by several cellular signaling pathways.

Mitogen activated protein kinases SakAHOG1 and MpkC collaborate for Aspergillus fumigatus virulence

It is proposed that both cell wall integrity (CWI) and HOG pathways collaborate, and that MpkC could act by modulating SakA activity upon exposure to several types of stresses and during CW biosynthesis.

Aspergillus fumigatus calcium-responsive transcription factors regulate cell wall architecture promoting stress tolerance, virulence and caspofungin resistance

ZipD-mediated regulation of the fungal cell wall contributes to the evasion of pro-inflammatory responses and tolerance of echinocandin antifungals, and in turn promoting virulence and complicating treatment options.

Aspergillus Fumigatus ZnfA, a Novel Zinc Finger Transcription Factor Involved in Calcium Metabolism and Caspofungin Tolerance

This work provides a detailed characterization of ZnfA, a transcription factor identified in previous screening studies that is involved in the A. fumigatus responses to calcium and CSP, and describes the existence of an additional, previously unknown TF that bridges calcium signaling and the CSP cellular response.

Signaling pathways for stress responses and adaptation in Aspergillus species: stress biology in the post-genomic era

Aspergillus species are among the most important filamentous fungi in terms of industrial use and because of their pathogenic or toxin-producing features. The genomes of several Aspergillus species

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