ChIP‐seq reveals a role for CrzA in the Aspergillus fumigatus high‐osmolarity glycerol response (HOG) signalling pathway

  title={ChIP‐seq reveals a role for CrzA in the Aspergillus fumigatus high‐osmolarity glycerol response (HOG) signalling pathway},
  author={Patr{\'i}cia A. Castro and Chenxi Chen and Ricardo S C Almeida and Fernanda Zanolli Freitas and Maria C{\'e}lia Bertolini and Enyara R Morais and Neil Andrew Brown and Leandra Naira Zambelli Ramalho and Daisuke Hagiwara and Thomas K. Mitchell and Gustavo Henrique Goldman},
  journal={Molecular Microbiology},
Aspergillus fumigatus is an opportunistic pathogen and allergen of mammals. Calcium signalling is essential for A. fumigatus pathogenicity and is regulated by the CrzA transcription factor. We used ChIP‐seq (Chromatin Immunoprecipitation DNA sequencing) to explore CrzA gene targets in A. fumigatus. In total, 165 potential binding peaks including 102 directly regulated genes were identified, resulting in the prediction of the A[GT][CG]CA[AC][AG] CrzA‐binding motif. The 102 CrzA putatively… 

The Aspergillus fumigatus Phosphoproteome Reveals Roles of High-Osmolarity Glycerol Mitogen-Activated Protein Kinases in Promoting Cell Wall Damage and Caspofungin Tolerance

This work investigates the global phosphoproteome of the ΔsakA and ΔmpkC A. fumigatus and high-osmolarity glycerol (HOG) pathway MAPK mutants upon cell wall damage to provide understanding of how fungal signal transduction networks modulate the cell wall.

Calcineurin‐dependent dephosphorylation of the transcription factor CrzA at specific sites controls conidiation, stress tolerance, and virulence of Aspergillus fumigatus

It is demonstrated that CrzA activity during calcium stimulation can only be suppressed by a high degree of phosphorylation in multiple regions of the protein, and the findings suggest that this regulation is not solely accomplished through control of CrZA nuclear import.

Elucidation of the calcineurin-Crz1 stress response transcriptional network in the human fungal pathogen Cryptococcus neoformans

The core calcineurin-Crz1 stress response cascade is maintained from ascomycetes to a pathogenic basidiomycete fungus, but its output in C. neoformans appears to be adapted to promote fungal virulence.

Aspergillus fumigatus High Osmolarity Glycerol Mitogen Activated Protein Kinases SakA and MpkC Physically Interact During Osmotic and Cell Wall Stresses

The hypothesis that SakA and MpkC are part of an osmotic and general signal pathways involved in regulation of the response to the cell wall damage, oxidative stress, drug resistance, and establishment of infection is supported.

The Aspergillus fumigatus SchASCH9 kinase modulates SakAHOG1 MAP kinase activity and it is essential for virulence

There is a complex network of interactions amongst the A. fumigatus TOR, SakA and SchA pathways, and Transcriptomics and proteomics identified direct and indirect targets of SchA post‐exposure to hyperosmotic stress.

The Aspergillus fumigatus CrzA Transcription Factor Activates Chitin Synthase Gene Expression during the Caspofungin Paradoxical Effect

The CPE is dependent on the cell wall integrity (CWI) mitogen-activated protein kinase MpkAMPK1 and its associated transcription factor (TF) RlmARLM1, which regulate chitin synthase gene expression in response to different concentrations of caspofungin, and shows that the regulation of cell wall biosynthetic genes occurs by several cellular signaling pathways.

Mitogen activated protein kinases SakAHOG1 and MpkC collaborate for Aspergillus fumigatus virulence

It is proposed that both cell wall integrity (CWI) and HOG pathways collaborate, and that MpkC could act by modulating SakA activity upon exposure to several types of stresses and during CW biosynthesis.

Aspergillus fumigatus calcium-responsive transcription factors regulate cell wall architecture promoting stress tolerance, virulence and caspofungin resistance

ZipD-mediated regulation of the fungal cell wall contributes to the evasion of pro-inflammatory responses and tolerance of echinocandin antifungals, and in turn promoting virulence and complicating treatment options.

Aspergillus Fumigatus ZnfA, a Novel Zinc Finger Transcription Factor Involved in Calcium Metabolism and Caspofungin Tolerance

This work provides a detailed characterization of ZnfA, a transcription factor identified in previous screening studies that is involved in the A. fumigatus responses to calcium and CSP, and describes the existence of an additional, previously unknown TF that bridges calcium signaling and the CSP cellular response.

Signaling pathways for stress responses and adaptation in Aspergillus species: stress biology in the post-genomic era

Aspergillus species are among the most important filamentous fungi in terms of industrial use and because of their pathogenic or toxin-producing features. The genomes of several Aspergillus species



NikA/TcsC Histidine Kinase Is Involved in Conidiation, Hyphal Morphology, and Responses to Osmotic Stress and Antifungal Chemicals in Aspergillus fumigatus

Although the signal transduction network related to NikA seems complicated, NikA plays a crucial role in several aspects of A. fumigatus physiology and, to a certain extent, modulates the HOG pathway.

Functional analysis of C2H2 zinc finger transcription factor CrzA involved in calcium signaling in Aspergillus nidulans

Comprehensive transcriptional analysis with DNA microarrays indicated that CrzA regulates the expression of a vacuolar Ca2+/H+ exchanger gene in response to external calcium stimuli, suggesting the importance of calcium homeostasis for adaptation to osmotic stress, a universal stress in filamentous fungi.

The SskA and SrrA Response Regulators Are Implicated in Oxidative Stress Responses of Hyphae and Asexual Spores in the Phosphorelay Signaling Network of Aspergillus nidulans

When examined these mutant strains under various conditions stressful for hyphal growth and asexual spore development, two of them showed a marked phenotype of hypersensitivity to oxidative stresses, indicative of the physiological importance of the His-Asp phosphorelay signaling network involving the SskA and SrrA response regulators.

Identification of possible targets of the Aspergillus fumigatus CRZ1 homologue, CrzA

A transcriptional profiling analysis of the A. fumigatus ΔAfcrzA mutant strain exposed to calcium stress provided an excellent opportunity to identify genes and pathways that are under the influence of AfCrzA.

Functional characterization of the Aspergillus fumigatus CRZ1 homologue, CrzA

This work characterized an Aspergillus fumigatus CRZ1 homologue, CrzA and demonstrated its mediation of cellular tolerance to increased concentrations of calcium and manganese, and linked calcineurin activity with asexual developmental induction, finding that CrZA supports appropriate developmental induction in a calcineURin and brlA‐dependent manner in both species.

The two-component histidine kinase Fhk1 controls stress adaptation and virulence of Fusarium oxysporum.

Fungal histidine kinases (HKs) have been implicated in different processes, such as the osmostress response, hyphal development, sensitivity to fungicides and virulence. Members of HK class III are

Combining ChIP-chip and Expression Profiling to Model the MoCRZ1 Mediated Circuit for Ca2+/Calcineurin Signaling in the Rice Blast Fungus

It is proposed that calcium/calcineurin regulated signal transduction circuits controlling development and pathogenicity manifest through multiple layers of regulation.

The histidine kinase CpHK2 has impact on spore germination, oxidative stress and fungicide resistance, and virulence of the ergot fungus Claviceps purpurea.

This is the first report of a role for a class X histidine kinase in a filamentous fungus, involved in spore germination, sensitivity to oxidative stress and fungicide resistance, in the biotrophic grass pathogen Claviceps purpurea.