ChIP‐Seq of ERα and RNA polymerase II defines genes differentially responding to ligands

@article{Welboren2009ChIPSeqOE,
  title={ChIP‐Seq of ER$\alpha$ and RNA polymerase II defines genes differentially responding to ligands},
  author={W. Welboren and M. V. van Driel and E. Janssen-Megens and S. V. van Heeringen and F. Sweep and P. Span and H. Stunnenberg},
  journal={The EMBO Journal},
  year={2009},
  volume={28}
}
We used ChIP‐Seq to map ERα‐binding sites and to profile changes in RNA polymerase II (RNAPII) occupancy in MCF‐7 cells in response to estradiol (E2), tamoxifen or fulvestrant. We identify 10 205 high confidence ERα‐binding sites in response to E2 of which 68% contain an estrogen response element (ERE) and only 7% contain a FOXA1 motif. Remarkably, 596 genes change significantly in RNAPII occupancy (59% up and 41% down) already after 1 h of E2 exposure. Although promoter proximal enrichment of… Expand
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