Certain carotenoids enhance the intracellular glutathione level in a murine cultured macrophage cell line by inducing glutamate-cysteine-ligase.

@article{Akaboshi2014CertainCE,
  title={Certain carotenoids enhance the intracellular glutathione level in a murine cultured macrophage cell line by inducing glutamate-cysteine-ligase.},
  author={Teppei Akaboshi and Rintaro Yamanishi},
  journal={Molecular nutrition & food research},
  year={2014},
  volume={58 6},
  pages={1291-300}
}
SCOPE Glutathione (GSH) increases in RAW264 murine macrophage cells exposed to β-carotene or β-cryptoxanthin, however, the underlying mechanism has not been clarified. In the present study, we investigated the expression of glutamate-cysteine-ligase (GCL), the rate-limiting enzyme in GSH synthesis, in these cells. METHODS AND RESULTS Both the protein and mRNA expression of GCL increased in a β-carotene concentration-dependent manner. Buthionine sulfoximine, a GCL inhibitor, abolished the… CONTINUE READING