Cerebrospinal fluid B cells from multiple sclerosis patients are subject to normal germinal center selection.

Abstract

Previous findings from our laboratory demonstrated that some clonally expanded cerebrospinal fluid (CSF) B cells from MS patients exhibit diminished mutation targeting patterns in comparison to typical B cells selected in the context of germinal centers (GCs). In order to determine whether the overall CSF B cell repertoires adhered to mutation patterns typical of GC-selected B cells, we analyzed the immunoglobulin repertoires from CSF B cells of 8 MS patients for mutation characteristics typical of GC-derived B cells. Mutation targeting was preserved. Thus, clonal expansion of some CSF B cells may occur independently of GC, but the CSF B cell pool is governed by typical GC selection. Interestingly, the heavy chain CDR3's of CSF B cells from MS patients had a net acidic charge, similar to GC-derived B cells, but a tendency towards longer CDR3's, consistent with autoreactive B cells. How these findings may support current hypotheses regarding the origin of CSF B cells is discussed.

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@article{Harp2007CerebrospinalFB, title={Cerebrospinal fluid B cells from multiple sclerosis patients are subject to normal germinal center selection.}, author={Christopher T Harp and Jane J. Lee and Doris Lambracht-Washington and Elizabeth M Cameron and Gregory Olsen and Elliot M . Frohman and Michael Karl Racke and Nancy Monson}, journal={Journal of neuroimmunology}, year={2007}, volume={183 1-2}, pages={189-99} }