Cereal Genomics: Methods and Protocols

@article{Henry2014CerealGM,
  title={Cereal Genomics: Methods and Protocols},
  author={R. Henry and A. Furtado},
  journal={Cereal Genomics},
  year={2014}
}
1. Dna Extraction from Vegetative Tissue for Next Generation Sequencing Agnelo Furtado 2. DNA Extraction from Rice Endosperm (Including a Protocol for Extraction of DNA from Ancient Seed Samples) Chiaki Mutou, Katsunori Tanaka, Ryuji Ishikawa 3. RNA Extraction from Cereal Vegetative Tissue Julie A. Pattemore 4. RNA Extraction from Developing or Mature Wheat Seeds Agnelo Furtado 5. cDNA Library Preparation Maarten Kooiker and Gang-Ping Xue 6. Preparation of High Molecular Weight gDNA and… Expand
8 Citations
Protocol: a simple method for extracting next-generation sequencing quality genomic DNA from recalcitrant plant species
TLDR
This protocol describes simple modifications to the established CTAB- based extraction method that allows for reliable isolation of high molecular weight genomic DNA from difficult to isolate plant species Corymbia and Coffea. Expand
A modular cloning toolkit for genome editing in plants
TLDR
An expanded cloning toolkit that contains modules encoding a variety of CRISPR/Cas-based nucleases and their corresponding guide RNA backbones and a set of modules that enable quick and facile assembly of tRNA-sgRNA polycistronic units without a PCR step involved is presented. Expand
Characterisation of an antimicrobial and phytotoxic ribonuclease secreted by the fungal wheat pathogen Zymoseptoria tritici
TLDR
The expression pattern of Zt6 and potent toxicity towards microorganisms suggests that whilst it may contribute to the execution of wheat cell death, it is also likely to have an important secondary function in antimicrobial competition and niche protection. Expand
The wheat Sr22, Sr33, Sr35 and Sr45 genes confer resistance against stem rust in barley
TLDR
Assessment of the functionality, in barley, of cloned wheat Sr genes effective against Ug99 found all four genes were found to confer effective stem rust resistance and are therefore a potential source of resistance against wheat stem rust in barley. Expand
Calmodulin HvCaM1 Negatively Regulates Salt Tolerance via Modulation of HvHKT1s and HvCAMTA41[OPEN]
TLDR
It is proposed that HvCaM1 is involved in regulating Na+ transport, probably via certain class I high-affinity potassium transporter (HvHKT1;5 and HvHK T1;1)-mediated Na+ translocation in roots, which may be a critical factor in the regulation of HKT1s in barley. Expand
Influence of Gene Expression on Hardness in Wheat
TLDR
The identification of wheat genotypes with hard grain texture and wild-type/soft Pin alleles indicating that hardness in wheat may be controlled by factors other than mutations in the coding region of the Pin genes is reported. Expand
Genomics of Food Grains
TLDR
Functional genomics and association genetics link genome sequence data to grain-quality attributes to study the study and utilization of grain foods. Expand
Transgene expression and plant regeneration in the Brassicaceae Aethionema arabicum
Transformation, der Einbau zusatzlicher DNA Abschnitte, ist eines der wichtigsten Werkzeuge in der modernen Molekularbiologie and Genetik, um Pflanzengenome modifizieren und folglich die Funktion undExpand

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