Centrosomal anchoring of protein kinase C betaII by pericentrin controls microtubule organization, spindle function, and cytokinesis.

@article{Chen2004CentrosomalAO,
  title={Centrosomal anchoring of protein kinase C betaII by pericentrin controls microtubule organization, spindle function, and cytokinesis.},
  author={Dan Chen and Aruna Purohit and Ensar Halilovic and Stephen John Doxsey and Alexandra C Newton},
  journal={The Journal of biological chemistry},
  year={2004},
  volume={279 6},
  pages={4829-39}
}
Location is a critical determinant in dictating the cellular function of protein kinase C (PKC). Scaffold proteins contribute to localization by poising PKC at specific intracellular sites. Using a yeast two-hybrid screen, we identified the centrosomal protein pericentrin as a scaffold that tethers PKC betaII to centrosomes. Co-immunoprecipitation studies reveal that the native proteins interact in cells. Co-overexpression studies show that the interaction is mediated by the C1A domain of PKC… CONTINUE READING