Centromeric Nucleosomes Induce Positive DNA Supercoils

  title={Centromeric Nucleosomes Induce Positive DNA Supercoils},
  author={Takehito Furuyama and Steven Henikoff},
Centromeres of higher eukaryotes are epigenetically maintained; however, the mechanism that underlies centromere inheritance is unknown. Centromere identity and inheritance require the assembly of nucleosomes containing the CenH3 histone variant in place of canonical H3. Although H3 nucleosomes wrap DNA in a left-handed manner and induce negative supercoils, we show here that CenH3 nucleosomes reconstituted from Drosophila histones induce positive supercoils. Furthermore, we show that CenH3… CONTINUE READING