Centromeric DNA of Kluyveromyces lactis

@article{Heus1990CentromericDO,
  title={Centromeric DNA of Kluyveromyces lactis},
  author={Joris Jan Heus and Ben J. M. Zonneveld and Herman Steensma and Johan A. van den Berg},
  journal={Current Genetics},
  year={1990},
  volume={18},
  pages={517-522}
}
A direct selection method was used to isolate centromeres from a genomic library of the yeast Kluyveromyces lactis. The method is based on the lethality at high copy number of the ochre-suppressing tRNA gene SUP11. Five different chromosomal fragments were found that confer mitotic stability to plasmids containing a replication origin of K. lactis (KARS). In addition, KARS plasmids containing these fragments have a copy number or approximately one, and each of the five fragments hybridizes to a… CONTINUE READING
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