Centromere formation in mouse cells cotransformed with human DNA and a dominant marker gene.

@article{Hadlaczky1991CentromereFI,
  title={Centromere formation in mouse cells cotransformed with human DNA and a dominant marker gene.},
  author={Gyula Hadlaczky and T{\"u}nde Praznovszky and Imre Cserp{\'a}n and Judit Keres{\"o} and Mikl{\'o}s P{\'e}terfy and I Kelemen and Erdal Atalay and Anna Szeles and J Szelei and V Tubak},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1991},
  volume={88 18},
  pages={8106-10}
}
A 13,863-base-pair (bp) putative centromeric DNA fragment has been isolated from a human genomic library by using a probe obtained from metaphase chromosomes of human colon carcinoma cells. The abundance of this DNA was estimated to be 16-32 copies per genome. Cotransfection of mouse cells with this sequence and a selectable marker gene (aminoglycoside 3'-phosphotransferase type II, APH-II) resulted in a transformed cell line carrying an additional centromere in a dicentric chromosome. This… CONTINUE READING

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