Centriole duplication in lysates of Spisula solidissima oocytes.

@article{Palazzo1992CentrioleDI,
  title={Centriole duplication in lysates of Spisula solidissima oocytes.},
  author={Robert E. Palazzo and Elena Vaisberg and R. W. Cole and Conly L. Rieder},
  journal={Science},
  year={1992},
  volume={256 5054},
  pages={
          219-21
        }
}
A cell-free system has been developed that executes centriole duplication. Surf clam (Spisula solidissima) oocytes, arrested at late prophase of meiosis I, do not contain centrioles, centrosomes, or asters. Serial section high-voltage electron microscopy (HVEM) of asters and spindles isolated from potassium chloride-activated oocytes indicates that within 4 minutes oocytes assemble a single centriole that is duplicated by 15 minutes when assembly of the first meiotic spindle is complete. A… CONTINUE READING

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