Centrin-2 Is Required for Centriole Duplication in Mammalian Cells

@article{Salisbury2002Centrin2IR,
  title={Centrin-2 Is Required for Centriole Duplication in Mammalian Cells},
  author={J. Salisbury and Kelly Suino and R. Busby and M. Springett},
  journal={Current Biology},
  year={2002},
  volume={12},
  pages={1287-1292}
}
BACKGROUND Centrosomes are the favored microtubule-organizing framework of eukaryotic cells. Centrosomes contain a pair of centrioles that normally duplicate once during the cell cycle to give rise to two mitotic spindle poles, each containing one old and one new centriole. However, aside from their role as an anchor point for pericentriolar material and as basal bodies of flagella and cilia, the functional attributes of centrioles remain enigmatic. RESULTS Here, using RNA interference, we… Expand
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TLDR
Fluorescence recovery after photobleaching experiments with green fluorescent protein fused to SAS-5 (GFP–SAS-5) demonstrated that the protein shuttles between centrioles and the cytoplasm throughout the cell cycle, and partial RNA-interference-mediated inactivation experiments suggest that both sas-5 and zyg-1 are dose-dependent regulators of centrosome duplication. Expand
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The authors concluded that the loss ofcentrioles was caused by a block at an early stage of centriole duplication, which led to defects in mitosis and cytokineses, and ultimately to cell death. Expand
Centriole assembly requires both centriolar and pericentriolar material proteins.
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A fluorescence microscopy-based assay for centriole assembly in the 1-cell stage C. elegans embryo is developed and shows that SAS-6, a member of a conserved metazoan protein family, is specifically required for new centrioles assembly, a result that is confirmed by electron microscopy. Expand
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One centrosome is reconstituted from two gametes at fertilization; in this case, the most common strategy involves differential contributions of centrioles and pericentriolar material (PCM) from each gamete. Expand
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TLDR
Centriole biology in general and especially in reproduction is reviewed, the discovery of the spermatozoon atypical centrioles is described, and an updated model for centriole inherence during sexual reproduction is provided. Expand
It takes two ( centrioles ) to tango Tomer
Cells that divide during embryo development require precisely two centrioles during interphase and four centrioles during mitosis. This precise number is maintained by allowing each centriole toExpand
Breaking Bad: Uncoupling of Modularity in Centriole Biogenesis and the Generation of Excess Centrioles in Cancer.
TLDR
The observations suggest that centriole replication is a modular, bottom-up process that is akin to building a house; the proper site of assembly is identified, a foundation is assembled at that site, and subsequent modules are added on top of the foundation. Expand
The roles of centrins/caltractins at the centrosome and in genome maintenance
TLDR
Notably, centrin-deficient DT40 cells undergo normal cellular division with no significant differences in cell cycle progression and microtubule re-nucleation analysis showed that the centrosomes retain function and cells are able to proceed through mitosis without any delay. Expand
Ab ovo or de novo? Mechanisms of centriole duplication
TLDR
Key proteins involved in centriole assembly are reviewed, two major modes of centrosome biogenesis are compared, and the mechanisms that ensure stringency of centrioles number are discussed. Expand
A novel human protein of the maternal centriole is required for the final stages of cytokinesis and entry into S phase
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It is concluded that centriolin is required for a late stage of vertebrate cytokinesis, perhaps the final cell cleavage event, and plays a role in progression into S phase. Expand
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