Central role of the oxygen-dependent degradation domain of Drosophila HIFalpha/Sima in oxygen-dependent nuclear export.

@article{Irisarri2009CentralRO,
  title={Central role of the oxygen-dependent degradation domain of Drosophila HIFalpha/Sima in oxygen-dependent nuclear export.},
  author={Maximiliano Irisarri and Sof{\'i}a Lavista-Llanos and Nuria Magdalena Romero and L{\'a}zaro Centanin and Andr{\'e}s Dekanty and Pablo Wappner},
  journal={Molecular biology of the cell},
  year={2009},
  volume={20 17},
  pages={
          3878-87
        }
}
The Drosophila HIFalpha homologue, Sima, is localized mainly in the cytoplasm in normoxia and accumulates in the nucleus upon hypoxic exposure. We have characterized the mechanism governing Sima oxygen-dependent subcellular localization and found that Sima shuttles continuously between the nucleus and the cytoplasm. We have previously shown that nuclear import depends on an atypical bipartite nuclear localization signal mapping next to the C-terminus of the protein. We show here that nuclear… CONTINUE READING