Central pore residues mediate the p97/VCP activity required for ERAD.

  title={Central pore residues mediate the p97/VCP activity required for ERAD.},
  author={Byron DeLaBarre and John C Christianson and Ron R. Kopito and Axel T Brunger},
  journal={Molecular cell},
  volume={22 4},
The AAA-ATPase p97/VCP facilitates protein dislocation during endoplasmic reticulum-associated degradation (ERAD). To understand how p97/VCP accomplishes dislocation, a series of point mutants was made to disrupt distinguishing structural features of its central pore. Mutants were evaluated in vitro for ATPase activity in the presence and absence of synaptotagmin I (SytI) and in vivo for ability to process the ERAD substrate TCRalpha. Synaptotagmin induces a 4-fold increase in the ATPase… CONTINUE READING
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Nucleotide dependent motion and mechanism of action of p97/VCP.

Journal of molecular biology • 2005
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A continuous spectrophotometric assay for inorganic phosphate and for measuring phosphate release kinetics in biological systems.

Proceedings of the National Academy of Sciences of the United States of America • 1992
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