Cellulose acetate electrophoretic determination of bone alkaline phosphatase activity in healthy subjects and diabetic patients with and without osteopenia.

Abstract

We adapted the electrophoretic method of bone alkaline phosphatase (ALP) determination using neuraminidase from Vibrio cholerae to separate bone and liver ALP on cellulose acetate membrane. Treatment of separator plus serum (1:8, neuraminidase 111 U/l in final) for 10 min at room temperature (25 +/- 1 degree C) and subsequent electrophoresis made it… (More)

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@article{Nishikawa1992CelluloseAE, title={Cellulose acetate electrophoretic determination of bone alkaline phosphatase activity in healthy subjects and diabetic patients with and without osteopenia.}, author={Y. Nishikawa and Tomonori Kanda and Harumasa Yoshihara and Kiyoko Fukumoto and Ikuo Uematsu}, journal={Clinica chimica acta; international journal of clinical chemistry}, year={1992}, volume={210 1-2}, pages={13-22} }