Cellulose Biosynthesis Inhibitors: Comparative Effect on Bean Cell Cultures

  title={Cellulose Biosynthesis Inhibitors: Comparative Effect on Bean Cell Cultures},
  author={Pen{\'e}lope Garc{\'i}a-Angulo and Ana Alonso-Sim{\'o}n and Antonio Encina and Jes{\'u}s Miguel {\'A}lvarez and Jos{\'e} Luis Acebes},
  journal={International Journal of Molecular Sciences},
  pages={3685 - 3702}
The variety of bioassays developed to evaluate different inhibition responses for cellulose biosynthesis inhibitors makes it difficult to compare the results obtained. This work aims (i) to test a single inhibitory assay for comparing active concentrations of a set of putative cellulose biosynthesis inhibitors and (ii) to characterize their effect on cell wall polysaccharides biosynthesis following a short-term exposure. For the first aim, dose-response curves for inhibition of dry-weight… Expand
It was hypothesized mixed linkage glucans (MLGs), a unique grass cell wall polysaccharide, have cell wall strengthening characteristic and may partially compensate for reduced cellulose content and support a conclusion that the noncellulosic fraction of grass primary cell walls has more load-bearing capacity than dicot cell walls. Expand
The biosynthesis and wall-binding of hemicelluloses in cellulose-deficient maize cells: an example of metabolic plasticity.
Cell-suspension cultures habituated to 2,6-dichlorobenzonitrile (DCB) survive with reduced cellulose owing to hemicellulose network modification are defined and habituated cells showed slower vesicular trafficking of polymers, especially xylans during logarithmic growth. Expand
Quinclorac-habituation of bean (Phaseolus vulgaris) cultured cells is related to an increase in their antioxidant capacity.
The results reported here indicate that the process of habituation to quinClorac in bean callus-cultured cells is related, at least partially, to the development of a stable antioxidant capacity that enables them to cope with the oxidative stress caused by quinclorac. Expand
Quinclorac is a systemic herbicide absorbed by germinating seeds, roots and leaves of seedlings. It is a selective compound for crops such as rice, canola, barley, corn, sorghum, and pasture.Expand
Investigation into the Cell Wall and Cellulose Biosynthesis in Model Species and in the C4 Model Plant Setaria viridis
A molecular genetics approach was taken to advance the understanding of cell wall biosynthesis and proposes a C4 model grass Setaria viridis as well as for the crop plant Sorghum bicolor for genetic analysis. Expand
Small but Mighty: An Update on Small Molecule Plant Cellulose Biosynthesis Inhibitors.
The effects of these CBIs on plant growth and development and plant cell biology are discussed, and what is known about the mode of action of these different CBIs is summarized. Expand
Alleles Causing Resistance to Isoxaben and Flupoxam Highlight the Significance of Transmembrane Domains for CESA Protein Function
Forward genetic screens in Arabidopsis revealed that mutations that can result in varying degrees of resistance to either isoxaben or flupoxam CBI can be attributed to single amino acid substitutions in primary wall CESAs, providing further genetic evidence supporting the involvement of CESA transmembrane regions in cellulose biosynthesis. Expand
Tolerancia adquirida en la habituación de cultivos de células de plantas a Diclobenil y Quinclorac: modificaciones en la pared celular y estrategia antioxidante
The structural plasticity of primary cell walls is proven, since a lignification process is possible in response to cellulose impoverishment. Expand
Teoria Integradora sobre o Modo de Ação de Quinclorac: Revisão de Literatura
It is theorized that the aforementioned biochemical activities are interconnected and can be the phytotoxic backbone to explain the herbicidal effect depending on the plant species and the plant growth stage, among other factors. Expand
Commentary: Hormesis can be used in enhancing plant productivity and health; but not as previously envisaged.
  • J. Gressel, J. Dodds
  • Biology, Medicine
  • Plant science : an international journal of experimental plant biology
  • 2013
Sub-toxic doses of many toxicants have positive, beneficial effects on productivity, or stress resistance (hormesis). Transcriptomic, proteomic, and metabolomic responses to a disparate varietyExpand


Characterization of a novel cellulose synthesis inhibitor
Freeze-fracture electron microscopy showed that the plasma membrane below the patterned thickenings of AE F150944-treated tracheary elements was depleted of cellulose-synthase-containing rosettes, which appeared to be inserted intact into the plasma membranes followed by their rapid disaggregation. Expand
Habituation of bean (Phaseolus vulgaris) cell cultures to Quinclorac and analysis of the subsequent cell wall modifications.
Long-term modifications of the cell wall caused by the habituation of bean cell cultures to quinclorac did not resemble those of bean cells habituated to the well-known cellulose biosynthesis inhibitors dichlobenil or isoxaben. Expand
Quinclorac does not inhibit cellulose (cell wall) biosynthesis in sensitive barnyard grass and maize roots
In contrast with previous reports, no evidence that quinclorac, directly or indirectly inhibits cellulose biosynthesis in roots of susceptible grasses was found. Expand
Ultrastructural effects of cellulose biosynthesis inhibitor herbicide on developing cotton fibers
The data indicate that all three herbicides are effective disrupters of cellulose biosynthesis and cause radical changes in cell wall structure and composition, and indicate that the composition of the walls may influence indirectly cell cycle kinetics, keeping these fiber cells in a more meristematic mode. Expand
A novel, cellulose synthesis inhibitory action of ancymidol impairs plant cell expansion
It is demonstrated here that ancymidol, known as a plant growth retardant primarily affecting gibberellin biosynthesis, is also capable of inhibiting cellulose synthesis. Expand
A novel thiazolidinone herbicide is a potent inhibitor of glucose incorporation into cell wall material
5-tert-Butyl-carbamoyloxy-3-(3-trifluoromethyl) phenyl-4-thiazolidinone (compound 1), a representative of a novel class of thiazolidinone herbicides, shows potential for the pre-emergence control ofExpand
Oxaziclomefone, a new herbicide, inhibits wall expansion in maize cell-cultures without affecting polysaccharide biosynthesis, xyloglucan transglycosylation, peroxidase action or apoplastic ascorbate oxidation.
OAC decreased wall extensibility without influencing the synthesis or post-synthetic modification of major architectural wall components, or the redox environment of the apoplast. Expand
Increase in the amount of celA1 protein in tobacco BY-2 cells by a cellulose biosynthesis inhibitor, 2,6-dichlorobenzonitrile.
The results suggest that celA1 protein is stabilized upon DCB binding and that the crystallization of cellulose microfibrils is inhibited simultaneously. Expand
Characterization of cell walls in bean (Phaseolus vulgaris L.) callus cultures tolerant to dichlobenil.
Cell wall fractionation showed that in tolerant cell walls the xyloglucan-cellulose network of non-tolerant cell walls was partly replaced by a pectin-rich network mainly formed of cross-linked polyuronides with a large proportion of homogalacturonan. Expand
Mechanism of Action and Selectivity of Quinclorac in Grass Roots
Results are consistent with a mechanism of action in which quinclorac acts as an inhibitor of cell wall biosynthesis in sensitive grasses, which could be explained by differential sensitivity at the site of action. Expand