Cellulase assays.

@article{Zhang2009CellulaseA,
  title={Cellulase assays.},
  author={Y H Percival Zhang and Jiong Hong and Xinhao Ye},
  journal={Methods in molecular biology},
  year={2009},
  volume={581},
  pages={
          213-31
        }
}
Cellulose is a heterogeneous polysaccharide, and its enzymatic hydrolysis requires endoglucanase, exoglucanase (cellobiohydrolase), and beta-glucosidase to work together. We summarize the most commonly used assays for individual enzymes and cellulase mixture. 
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Lignocellulose-degrading fungi are considered as the most promising strategy for biomass utilization. In this work two different fungal isolates were investigated for their ability to produce…
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References

SHOWING 1-10 OF 24 REFERENCES
Saccharification of Complex Cellulosic Substrates by the Cellulase System from Clostridium thermocellum
TLDR
True cellulase activity has been demonstrated in cell-free preparations from the thermophilic anaerobe Clostridium thermocellum and the absence of substrate at temperatures up to 70 degrees C, carboxymethylcellulase was much more unstable than the Avicel-hydrolyzing activity.
Affinity digestion for the near-total recovery of purified cellulosome from Clostridium thermocellum
Toward an aggregated understanding of enzymatic hydrolysis of cellulose: Noncomplexed cellulase systems
TLDR
It is suggested that it is timely to revisit and reinvigorate functional modeling of cellulose hydrolysis and that this would be highly beneficial if not necessary in order to bring to bear the large volume of information available on cellulase components on the primary applications that motivate interest in the subject.
A functionally based model for hydrolysis of cellulose by fungal cellulase
TLDR
This is the first cellulase kinetic model involving a single set of kinetic parameters that is successfully applied to a variety of cellulosic substrates, and the first that describes more than one behavior associated with enzymatic hydrolysis.
Outlook for cellulase improvement: screening and selection strategies.
Microbial Cellulose Utilization: Fundamentals and Biotechnology
TLDR
A concluding discussion identifies unresolved issues pertaining to microbial cellulose utilization, suggests approaches by which such issues might be resolved, and contrasts a microbially oriented cellulose hydrolysis paradigm to the more conventional enzymatically oriented paradigm in both fundamental and applied contexts.
Regulation of Cellulase Synthesis in Batch and Continuous Cultures of Clostridium thermocellum
TLDR
The differences between the cellul enzyme yields observed during carbon-limited growth on cellulose and the cellulase yields observed in Avicel-grown batch cultures at the same dilution rate suggest that hydrolysis products other than cellobiose affect cellulase synthesis during growth on Cellobiose and/or that the presence of insoluble cellulose triggers an increase in cellul enzyme synthesis.
METHODS FOR MEASURING CELLULASE ACTIVITIES
Quantification of cell and cellulase mass concentrations during anaerobic cellulose fermentation: development of an enzyme-linked immunosorbent assay-based method with application to Clostridium thermocellum batch cultures.
TLDR
It is concluded that the reported protocols establish a reasonable methodological basis for quantitative determination of the mass concentration of cellulase protein produced by C. thermocellum and for calculation of cell mass concentration as distinct from cellulase concentration.
Bioseparation of recombinant cellulose-binding module-proteins by affinity adsorption on an ultra-high-capacity cellulosic adsorbent.
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